Browse by Aston Author

2024

de Vries, Tessa, Labruijere, Sieneke, Rivera‐Mancilla, Eduardo, Garrelds, Ingrid M., de Vries, René, Schutter, Dennis, van den Bogaerdt, Antoon, Poyner, David R., Ladds, Graham, Danser, A. H. Jan and MaassenVanDenBrink, Antoinette (2024). Intracellular pathways of calcitonin gene‐related peptide‐induced relaxation of human coronary arteries:A key role for Gβγ subunit instead of cAMP. British Journal of Pharmacology, 181 (15), pp. 2478-2491.

Ayub, Hoor, Murray, Rebecca J., Kuyler, Gestél C., Napier-Khwaja, Farhaan, Gunner, Joseph, Dafforn, Tim R., Klumperman, Bert, Poyner, David R. and Wheatley, Mark (2024). GPCRs in the round:SMA-like copolymers and SMALPs as a platform for investigating GPCRs. Archives of Biochemistry and Biophysics, 754 ,

2022

Pearce, Abigail, Redfern-Nichols, Theo, Harris, Matthew, Poyner, David R., Wigglesworth, Mark and Ladds, Graham (2022). Determining the Effects of Differential Expression of GRKs and β-arrestins on CLR-RAMP Agonist Bias. Frontiers in Physiology, 13 ,

Winfield, Ian, Barkan, Kerry, Routledge, Sarah, Robertson, Nathan J., Harris, Matthew, Jazayeri, Ali, Simms, John, Reynolds, Christopher A., Poyner, David R. and Ladds, Graham (2022). The Role of ICL1 and H8 in Class B1 GPCRs; Implications for Receptor Activation. Frontiers in Endocrinology, 12 ,

2021

Harwood, C.R., Sykes, D.A., Hoare, B., Heydenreich, F.M., Uddin, R., Poyner, D.R., Briddon, S.J. and Veprintsev, D.B. (2021). Functional solubilization of the β 2-adrenoceptor using diisobutylene maleic acid. iScience, 24 (12),

Grime, Rachael L., Logan, Richard T., Nestorow, Stephanie A., Sridhar, Pooja, Edwards, Patricia C., Tate, Christopher G., Klumperman, Bert, Dafforn, Tim R., Poyner, David R., Reeves, Philip J. and Wheatley, Mark (2021). Differences in SMA-like polymer architecture dictate the conformational changes exhibited by the membrane protein rhodopsin encapsulated in lipid nano-particles. Nanoscale, 13 (31), pp. 13519-13528.

Clark, Ashley, Mullooly, Niamh, Safitri, Dewi, Harris, Matthew, de Vries, Tessa, Maassen Van Den Brink, Antoinette, Poyner, David, Gianni, Davide, Wrigglesworth, Mark and Ladds, Graham (2021). CGRP, adrenomedullin and adrenomedullin 2 display endogenous GPCR agonist bias in primary human cardiovascular cells. Communications Biology, 4 ,

2020

Gulamhussein, Aiman, Uddin, Romez, Tighe, Brian, Poyner, David and Rothnie, Alice (2020). A comparison of SMA (styrene maleic acid) and DIBMA (di-isobutylene maleic acid) for membrane protein purification. BBA -Biomembranes, 1862 (7),

Grime, Rachael L., Goulding, Joelle, Uddin, Romez, Stoddart, Leigh A., Hill, Stephen J., Poyner, David, Briddon, S. and Wheatley, Mark (2020). Single molecule binding of a ligand to a G-protein-coupled receptor in real time using fluorescence correlation spectroscopy, rendered possible by nano-encapsulation in styrene maleic acid lipid particles. Nanoscale, 12 (21), pp. 11518-11525.

Routledge, Sarah J., Jamshad, Mohammed, Little, Haydn A., Lin, Yu Pin, Simms, John, Thakker, Alpesh, Spickett, Corinne M., Bill, Roslyn M., Dafforn, Tim R., Poyner, David R. and Wheatley, Mark (2020). Ligand-induced conformational changes in a SMALP-encapsulated GPCR. Biochimica et Biophysica Acta - Biomembranes, 1862 (6),

Lee, Sang-min, Jeong, Yejin, Simms, John, Warner, Margaret L., Poyner, David R., Chung, Ka Young and Pioszak, Augen A. (2020). Calcitonin receptor N-glycosylation enhances peptide hormone affinity by controlling receptor dynamics. Journal of Molecular Biology, 432 (7), pp. 1996-2014.

Wiseman, Daniel N., Otchere, Abigail, Patel, Jaimin H., Uddin, Romez, Pollock, Naomi L., Routledge, Sarah J., Rothnie, Alice J., Slack, Cathy, Poyner, David R., Bill, Roslyn M. and Goddard, Alan D. (2020). Expression and purification of recombinant G protein-coupled receptors: A review. Protein Expression and Purification, 167 ,

Routledge, Sarah J., Simms, John, Clark, Ashley, Yeung, Ho Yan, Wigglesworth, Mark J., Dickerson, Ian M., Kitchen, Philip, Ladds, Graham and Poyner, David R. (2020). Receptor component protein, an endogenous allosteric modulator of family B G protein coupled receptors. BBA -Biomembranes, 1862 (3),

2019

Bailey, Sian, Harris, Matthew, Barkan, Kerry, Winfield, Ian, Thomas-Harper, Matthew, Simms, John W, Ladds, Graham, Wheatley, Mark and Poyner, David R (2019). Interactions Between RAMP2 And CRF Receptors:The Effect Of Receptor Subtypes, Splice Variants And Cell Context. BBA -Biomembranes, 1861 (5), pp. 997-1003.

2018

Uddin, Romez, Simms, John and Poyner, David (2018). Functional characterisation of G protein-coupled receptors. Methods, 147 , pp. 213-220.

Dilworth, Marvin V., Piel, Mathilde S., Bettaney, Kim E., Ma, Pikyee, Luo, Ji, Sharples, David, Poyner, David R., Gross, Stephane R., Moncoq, Karine, J. F. Henderson, Peter, Miroux, Bruno and Bill, Roslyn M. (2018). Microbial expression systems for membrane proteins. Methods, 147 , pp. 3-39.

Mackie, Duncan I., Al Mutairi, Fuad, Davis, Reema B., Kechele, Daniel O., Nielsen, Natalie R., Snyder, Joshua C., Caron, Marc G., Kliman, Harvey J., Berg, Jonathan S., Simms, John, Poyner, David R. and Caron, Kathleen M. (2018). hCALCRL mutation causes autosomal recessive nonimmune hydrops fetalis with lymphatic dysplasia. Journal of Experimental Medicine, 215 (9), pp. 2339-2353.

Simms, John, Uddin, Romez, Sakmar, Thomas P., Gingell, Joseph J., Garelja, Michael L., Hay, Debbie L., Brimble, Margaret A., Harris, Paul W. R., Reynolds, Christopher Arthur and Poyner, David R. (2018). Photoaffinity cross-linking and unnatural amino acid mutagenesis reveal insights into calcitonin gene-related peptide binding to the calcitonin receptor-like receptor/receptor activity-modifying protein 1 (CLR/RAMP1) complex. Biochemistry, 57 (32), pp. 4915-4922.

2017

Hay, Debbie L., Garelja, Michael L., Poyner, David R and Walker, Christopher S. (2017). Update on the pharmacology of calcitonin/CGRP family of peptides:IUPHAR Review 25. British Journal of Pharmacology, 175 (1), pp. 3-17.

Woolley, Michael J., Reynolds, Christopher A., Simms, John, Walker, Christopher S., Mobarec, Juan Carlos, Garelja, Michael L., Conner, Alex C., Poyner, David R. and Hay, Debbie L. (2017). Receptor activity-modifying protein dependent and independent activation mechanisms in the coupling of calcitonin gene-related peptide and adrenomedullin receptors to Gs. Biochemical Pharmacology, 142 , pp. 96-110.

Woolley, Michael J., Simms, John, Mobarec, Juan Carlos, Reynolds, Christopher A., Poyner, David R. and Conner, Alex C. (2017). Understanding the molecular functions of the second extracellular loop (ECL2) of the calcitonin gene-related peptide (CGRP) receptor using a comprehensive mutagenesis approach. Molecular and Cellular Endocrinology, 454 , pp. 39-49.

Woolley, Michael J., Simms, John, Uddin, Sifat, Poyner, David R. and Conner, Alex C. (2017). Relative antagonism of mutants of the CGRP receptor extracellular loop 2 domain (ECL2) using a truncated competitive antagonist (CGRP8-37):evidence for the dual involvement of ECL2 in the two-domain binding model. Biochemistry, 56 (30), pp. 3877-3880.

Routledge, Sarah J., Ladds, Graham and Poyner, David R. (2017). The effects of RAMPs upon cell signalling. Molecular and Cellular Endocrinology, 449 , pp. 12-20.

2016

Weston, Cathryn, Winfield, Ian, Harris, Matthew, Hodgson, Rose, Shah, Archna, Dowell, Simon J., Mobarec, Juan Carlos, Woodlock, David A., Reynolds, Christopher A., Poyner, David R., Watkins, Harriet A. and Ladds, Graham (2016). Receptor activity-modifying protein-directed G protein signaling specificity for the calcitonin gene-related peptide family of receptors. Journal of Biological Chemistry, 291 (42), pp. 21925-21944.

Watkins, Harriet A., Chakravarthy, Madhuri, Abhayawardana, Rekhati S., Gingell, Joseph J., Garelja, Michael, Pardamwar, Meenakshi, McElhinney, James M.W.R., Lathbridge, Alex, Constantine, Arran, Harris, Paul W.R., Yuen, Tsz-Ying, Brimble, Margaret A., Barwell, James, Poyner, David R., Woolley, Michael J., Conner, Alex C., Pioszak, Augen A., Reynolds, Christopher A. and Hay, Debbie L. (2016). Receptor activity-modifying proteins 2 and 3 generate adrenomedullin receptor subtypes with distinct molecular properties. Journal of Biological Chemistry, 291 (22), pp. 11657-11675.

Gingell, Joseph J., Simms, John, Barwell, James, Poyner, David R., Watkins, Harriet A., Pioszak, Augen A., Sexton, Patrick M. and Hay, Debbie L. (2016). An allosteric role for receptor activity-modifying proteins in defining GPCR pharmacology. Cell Discovery, 2 ,

Wheatley, Mark, Charlton, Jack, Jamshad, Mohammed, Routledge, Sarah J., Bailey, Sian, La-Borde, Penelope J., Azam, Maria T., Logan, Richard T., Bill, Roslyn M., Dafforn, Tim R. and Poyner, David R. (2016). GPCR-styrene maleic acid lipid particles (GPCR-SMALPs):their nature and potential. Biochemical Society Transactions, 44 (2), pp. 619-623.

2015

Weston, Cathryn, Lu, Jing, Li, Naichang, Barkan, Kerry, Richards, Gareth O., Roberts, David J., Skerry, Timothy M., Poyner, David, Pardamwar, Meenakshi, Reynolds, Christopher A., Dowell, Simon J., Willars, Gary B. and Ladds, Graham (2015). Modulation of glucagon receptor pharmacology by Receptor Activity-modifying Protein-2 (RAMP2). Journal of Biological Chemistry, 290 (38), pp. 23009-23022.

Booe, Jason M., Walker, Christopher S., Barwell, James, Kuteyi, Gabriel, Simms, John, Jamaluddin, Muhammad A., Warner, Margaret L., Bill, Roslyn M., Harris, Paul W., Brimble, Margaret A., Poyner, David R., Hay, Debbie L. and Pioszak, Augen A. (2015). Structural basis for receptor activity-modifying protein-dependent selective peptide recognition by a G protein-coupled receptor. Molecular Cell, 58 (6), pp. 1040-1052.

Jamshad, Mohammed, Charlton, Jack, Lin, Yu-Pin, Routledge, Sarah J, Bawa, Zharain, Knowles, Timothy J., Overduin, Michael, Dekker, Niek, Dafforn, Tim R., Bill, Roslyn M., Poyner, David R. and Wheatley, Mark (2015). G-protein-coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent. Bioscience Reports, 35 (2),

2014

Bawa, Zharain, Routledge, Sarah J., Jamshad, Mohammed, Clare, Michelle, Sarkar, Debasmita, Dickerson, Ian, Ganzlin, Markus, Poyner, David R. and Bill, Roslyn M. (2014). Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks. Microbial Cell Factories, 13 (1),

Weston, C., Poyner, D., Patel, V., Dowell, S. and Ladds, G. (2014). Investigating G protein signalling bias at the glucagon-like peptide-1 receptor in yeast. British Journal of Pharmacology, 171 (15), pp. 3651-3665.

Watkins, H.A., Walker, C.S., Ly, K.N., Bailey, R.J., Barwell, J., Poyner, D.R. and Hay, D.L. (2014). Receptor activity-modifying protein-dependent effects of mutations in the calcitonin receptor-like receptor:implications for adrenomedullin and calcitonin gene-related peptide pharmacology. British Journal of Pharmacology, 171 (3), pp. 772-788.

Routledge, Sarah J., Poyner, David R. and Bill, Roslyn M. (2014). Antifoams:the overlooked additive? Pharmaceutical Bioprocessing, 2 (2), pp. 103-106.

Hay, Debbie L., Harris, Paul W.R., Kowalczyk, Renata, Brimble, Margaret A., Rathbone, Dan L, Barwell, James, Conner, Alex C. and Poyner, David R. (2014). Structure-activity relationships of the N-terminus of calcitonin gene-related peptide:key roles of alanine-5 and threonine-6 in receptor activation. British Journal of Pharmacology, 171 (2), pp. 415-426.

2013

Woolley, Michael J., Watkins, Harriet A., Taddese, Bruck, Karakullukcu, Z. Gamze, Barwell, James, Smith, Kevin J., Hay, Debbie L., Poyner, David R., Reynolds, Christopher A. and Conner, Alex C. (2013). The role of ECL2 in CGRP receptor activation:a combined modelling and experimental approach. Journal of the Royal Society Interface, 10 (88),

Vohra, Shabana, Taddese, Bruck, Conner, Alex C., Poyner, David R., Hay, Debbie L., Barwell, James, Reeves, Philip J., Upton, Graham J.G. and Reynolds, Christopher A. (2013). Similarity between class A and class B G-protein-coupled receptors exemplified through calcitonin gene-related peptide receptor modelling and mutagenesis studies. Journal of the Royal Society Interface, 10 (79),

2011

Barwell, James, Conner, Alex C. and Poyner, David R (2011). Extracellular loops 1 and 3 and their associated transmembrane regions of the calcitonin receptor-like receptor are needed for CGRP receptor function. BBA - Molecular Cell Research, 1813 (10), pp. 1906-1916.

2010

Miller, Philip S., Barwell, James, Poyner, David R., Wigglesworth, Mark J., Garland, Stephen L. and Donnelly, Dan (2010). Non-peptidic antagonists of the CGRP receptor, BIBN4096BS and MK-0974, interact with the calcitonin receptor-like receptor via methionine-42 and RAMP1 via tryptophan-74. Biochemical and Biophysical Research Communications, 391 (1), pp. 437-442.

Barwell, James, Miller, Philip S., Donnelly, Dan and Poyner, David R. (2010). Mapping interaction sites within the N-terminus of the calcitonin gene-related peptide receptor; the role of residues 23-60 of the calcitonin receptor-like receptor. Peptides, 31 (1), pp. 170-176.

2009

Bonander, Nicklas, Darby, Richard A.J., Grgic, Ljuban, Bora, Nagamani, Wen, Jikai, Brogna, Saverio, Poyner, David R., O'Neill, Michael A. A. and Bill, Roslyn M. (2009). Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield. Microbial Cell Factories, 8 (10),

2006

Conner, Alex C., Simms, John, Howitt, Stephen G., Wheatley, Mark and Poyner, David R. (2006). The second intracellular loop of the calcitonin gene-related peptide receptor provides molecular determinants for signal transduction and cell surface expression. Journal of Biological Chemistry, 281 (3), pp. 1644-1651.

1993

Poyner, David R., Cooke, F., Hanley, Michael R., Reynolds, D.J. and Hawkins, Phillip T. (1993). Characterization of metal ion-induced [3H]inositol hexakisphosphate binding to rat cerebellar membranes. Journal of Biological Chemistry, 268 (2), pp. 1032-1038.