Calcitonin receptor N-glycosylation enhances peptide hormone affinity by controlling receptor dynamics


The class B G protein-coupled receptor (GPCR) calcitonin receptor (CTR) is a drug target for osteoporosis and diabetes. N-glycosylation of asparagine 130 in its extracellular domain (ECD) enhances calcitonin hormone affinity with the proximal GlcNAc residue mediating this effect through an unknown mechanism. Here, we present two crystal structures of salmon calcitonin-bound, GlcNAc-bearing CTR ECD at 1.78 and 2.85 Å resolutions and analyze the mechanism of the glycan effect. The N130 GlcNAc does not contact the hormone. Surprisingly, the structures are nearly identical to a structure of hormone-bound, N-glycan-free ECD, which suggested that the GlcNAc might affect CTR dynamics not observed in the static crystallographic snapshots. Hydrogen-deuterium exchange mass spectrometry and molecular dynamics simulations revealed that glycosylation stabilized a β-sheet adjacent to the N130 GlcNAc and the N-terminal α-helix near the peptide-binding site, while increasing flexibility of the peptide-binding site turret loop. These changes due to N-glycosylation increased the ligand on-rate and decreased its off rate. The glycan effect extended to RAMP-CTR amylin receptor complexes and was also conserved in the related CGRP receptor. These results reveal that N-glycosylation can modulate GPCR function by altering receptor dynamics.

Publication DOI:
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences > Aston Pharmacy School
Additional Information: © 2020 The Author(s). CC BY Funding: NIH grant R01GM104251 to AAP, the National Research Foundation of Korea grant NRF-2018R1A2B6001554 to KYC, and use of the crystal imaging microscope was supported by NIH COBRE award P20GM103640. DRP and JS were supported by the BBSRC grant BB/M007529/1.
Uncontrolled Keywords: G protein-coupled receptor (GPCR),N-linked glycosylation,dynamic allostery,ligand binding kinetics,peptide hormone,Structural Biology,Molecular Biology
Publication ISSN: 0022-2836
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Related URLs: https://linking ... 022283620300929 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2020-03-27
Published Online Date: 2020-02-06
Accepted Date: 2020-01-27
Authors: Lee, Sang-min
Jeong, Yejin
Simms, John (ORCID Profile 0000-0002-4675-0902)
Warner, Margaret L.
Poyner, David R. (ORCID Profile 0000-0003-1590-112X)
Chung, Ka Young
Pioszak, Augen A.



Version: Accepted Version

License: Creative Commons Attribution

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