Wiseman, Daniel N., Otchere, Abigail, Patel, Jaimin H., Uddin, Romez, Pollock, Naomi L., Routledge, Sarah J., Rothnie, Alice J., Slack, Cathy, Poyner, David R., Bill, Roslyn M. and Goddard, Alan D. (2020). Expression and purification of recombinant G protein-coupled receptors: A review. Protein Expression and Purification, 167 ,
Abstract
Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available prophylaxis. Novel drug design and discovery significantly rely on structure determination, of which GPCRs are typically elusive. Progress has been made thus far to produce sufficient quantity and quality of protein for downstream analysis. As such, this review highlights the systems available for recombinant GPCR expression, with consideration of their advantages and disadvantages, as well as examples of receptors successfully expressed in these systems. Additionally, an overview is given on the use of detergents and the styrene maleic acid (SMA) co-polymer for membrane solubilisation, as well as purification techniques.
Publication DOI: | https://doi.org/10.1016/j.pep.2019.105524 |
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Divisions: | College of Health & Life Sciences > School of Biosciences College of Health & Life Sciences > Aston Pharmacy School College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine College of Health & Life Sciences Aston University (General) |
Funding Information: | This project has received funding from the European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement No 847419 Funding: We are grateful for support from BBSRC (BB/N007417/1, BB/P025927/1, BB/P022685/1, BB |
Additional Information: | Funding: We are grateful for support from BBSRC (BB/N007417/1, BB/P025927/1, BB/P022685/1, BB/M016668/1, BB/L015846/1, BB/M007529/1, BB/R02152X/1, BB/S004696/1 and BB/T001488/1), H2020 ERA CoBioTech (under grant agreement No. 722361) and H2020 MSCA COFUND (under grant agreement No. 847419). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement No 847419 © 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/). |
Uncontrolled Keywords: | Expression,GPCR,Purification,Review,SMALP,Biotechnology |
Publication ISSN: | 1096-0279 |
Last Modified: | 25 Nov 2024 08:19 |
Date Deposited: | 04 Nov 2019 13:56 |
Full Text Link: | |
Related URLs: |
https://linking ... 046592819304930
(Publisher URL) http://www.scop ... tnerID=8YFLogxK (Scopus URL) |
PURE Output Type: | Article |
Published Date: | 2020-03-01 |
Published Online Date: | 2019-10-31 |
Accepted Date: | 2019-10-29 |
Authors: |
Wiseman, Daniel N.
(
0000-0001-5614-5581)
Otchere, Abigail Patel, Jaimin H. Uddin, Romez Pollock, Naomi L. Routledge, Sarah J. Rothnie, Alice J. ( 0000-0002-4259-7015) Slack, Cathy ( 0000-0002-7949-4079) Poyner, David R. ( 0000-0003-1590-112X) Bill, Roslyn M. ( 0000-0003-1331-0852) Goddard, Alan D. ( 0000-0003-4950-7470) |
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