In Vitro and In Silico Investigation of Water-Soluble Fullerenol C60(OH)24:Bioactivity and Biocompatibility

Abstract

Light fullerenes, C60 and C70, have significant potential in biomedical applications due to their ability to absorb reactive oxygen species, inhibit the development of tumors, inactivate viruses and bacteria, and as the basis for developing systems for targeted drug delivery. However, the hydrophobicity of individual fullerenes complicates their practical use; therefore, creating water-soluble derivatives of fullerenes is increasingly important. Currently, the most studied soluble adducts of fullerenes are polyhydroxy fullerenes or fullerenols. Unfortunately, investigations of fullerenol biocompatibility are fragmental. They often lack reproducibility both in the synthesis of the compounds and their biological action. We here investigate the biocompatibility of a well-defined fullerenol C60(OH)24 obtained using methods that minimize the content of impurities and quantitatively characterize the product’s composition. We carry out comprehensive biochemical and biophysical investigations of C60(OH)24 that include photodynamic properties, cyto- and genotoxicity, hemocompatibility (spontaneous and photo-induced hemolysis, platelet aggregation), and the thermodynamic characteristics of C60(OH)24 binding to human serum albumin and DNA. The performed studies show good biocompatibility of fullerenol C60(OH)24, which makes it a promising object for potential use in biomedicine.

Publication DOI: https://doi.org/10.1021/acs.jpcb.1c03332
Divisions: College of Engineering & Physical Sciences > Aston Institute of Urban Technology and the Environment (ASTUTE)
College of Engineering & Physical Sciences > Systems analytics research institute (SARI)
College of Engineering & Physical Sciences
Funding Information: This work was supported by a grant from the Russian Science Foundation (20-79-10064). The research was performed using the equipment of the Resource Center “GeoModel”, Center for Chemical Analysis and Materials Research, Interdisciplinary Resource Center
Additional Information: This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry: Part B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jpcb.1c03332 Funding: This work was supported by a grant from the Russian Science Foundation (20-79-10064).
Uncontrolled Keywords: Physical and Theoretical Chemistry,Surfaces, Coatings and Films,Materials Chemistry
Publication ISSN: 1520-5207
Last Modified: 16 Apr 2024 07:34
Date Deposited: 02 Sep 2021 10:57
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://pubs.ac ... cs.jpcb.1c03332 (Publisher URL)
PURE Output Type: Article
Published Date: 2021-08-19
Published Online Date: 2021-08-10
Accepted Date: 2021-07-21
Authors: Sharoyko, Vladimir V.
Iamalova, Nailia R.
Ageev, Sergei V.
Meshcheriakov, Anatolii A.
Iurev, Gleb O.
Petrov, Andrey V.
Nerukh, Dmitry A. (ORCID Profile 0000-0001-9005-9919)
Farafonov, Vladimir S.
Vasina, Lubov V.
Penkova, Anastasia V.
Semenov, Konstantin N.

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