Scale-up of an intensified bioprocess for the expansion of bovine adipose-derived stem cells (bASCs) in stirred tank bioreactors

Abstract

Cultivated meat is an emerging field, aiming to establish the production of animal tissue for human consumption in an in vitro environment, eliminating the need to raise and slaughter animals for their meat. To realise this, the expansion of primary cells in a bioreactor is needed to achieve the high cell numbers required. The aim of this study was to develop a scalable, microcarrier based, intensified bioprocess for the expansion of bovine adipose-derived stem cells as precursors of fat and muscle tissue. The intensified bioprocess development was carried out initially in spinner flasks of different sizes and then translated to fully controlled litre scale benchtop bioreactors. Bioprocess intensification was achieved by utilising the previously demonstrated bead-to-bead transfer phenomenon and through the combined addition of microcarrier and medium to double the existing surface area and working volume in the bioreactor. Choosing the optimal time point for the additions was critical in enhancing the cell expansion. A significant fold increase of 114.19 ± 1.07 was obtained at the litre scale in the intensified bioprocess compared to the baseline (**p < .005). The quality of the cells was evaluated pre- and post-expansion and the cells were found to maintain their phenotype and differentiation capacity.

Publication DOI: https://doi.org/10.1002/bit.27842
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
Additional Information: © 2021 Biotechnology and Bioengineering published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Funding: The Good Food Institute (US) (Grant Number(s): 2018 GFI Competitive Grant Program)
Uncontrolled Keywords: bioprocessing,bioreactors,cultivated beef,microcarriers,scale-up,Biotechnology,Bioengineering,Applied Microbiology and Biotechnology
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Related URLs: https://onlinel ... .1002/bit.27842 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2021-08
Published Online Date: 2021-06-02
Accepted Date: 2021-05-18
Authors: Hanga, Mariana P. (ORCID Profile 0000-0002-2427-4052)
de la Raga, Fritz Anthony
Moutsatsou, Panagiota
Hewitt, Christopher
Nienow, Alvin
Wall, Ivan (ORCID Profile 0000-0001-6294-8348)

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