In vitro and in vivo osteogenic potential of niobium-doped 45S5 bioactive glass:A comparative study


In vitro and in vivo experiments were undertaken to evaluate the solubility, apatite-forming ability, cytocompatibility, osteostimulation, and osteoinduction for a series of Nb-containing bioactive glass (BGNb) derived from composition of 45S5 Bioglass. Inductively coupled plasma optical emission spectrometry (ICP-OES) revealed that the rate at which Na, Ca, Si, P, and Nb species are leached from the glass decrease with the increasing concentration of the niobium oxide. The formation of apatite as a function of time in simulated body fluid was monitored by 31P Magic Angle Spinning (MAS) Nuclear magnetic resonance spectroscopy. Results showed that the bioactive glasses: Bioglass 45S5 (BG45S5) and 1 mol%-Nb-containing-bioactive glass (BGSN1) were able to grow apatite layer on their surfaces within 3 h, while glasses with higher concentrations of Nb2O5 (2.5 and 5 mol%) took at least 12 h. Nb-substituted glasses were shown to be compatible with bone marrow-derived mesenchymal stem cells (BMMSCs). Moreover, the bioactive glass with 1 mol% Nb2O5 significantly enhanced cell proliferation after 4 days of treatment. Concentrations of 1 and 2.5 mol% Nb2O5 stimulated osteogenic differentiation of BMMSCs after 21 days of treatment. For the in vivo experiments, trial glass rods were implanted into circular defects in rat tibia in order to evaluate their osteoconductivity and osteostimulation. Two morphometric parameters were analyzed: (a) thickness of new-formed bone layer and (b) area of new-formed subperiostal bone. Results showed that BGNb bioactive glass is osteoconductive and osteostimulative. Therefore, these results indicate that Nb-substituted glass is suitable for biomedical applications.

Publication DOI:
Divisions: College of Engineering & Physical Sciences > Aston Institute of Materials Research (AIMR)
College of Engineering & Physical Sciences
Funding Information: The authors acknowledge the use of the analytical instrumentation facility at the Institute of Chemistry, the University of Campinas, which is supported by the State of Paulo. This work was carried out with the support of the São Paulo Research Foundation
Additional Information: This is the peer reviewed version of the following article: Lopes, JH, Souza, LP, Domingues, JA, et al. In vitro and in vivo osteogenic potential of niobium‐doped 45S5 bioactive glass: A comparative study. J Biomed Mater Res. 2019; 1– 16, which has been published in final form at  This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Uncontrolled Keywords: bioactive glass,bone regeneration,niobium,osteoinduction,osteostimulation,Biomaterials,Biomedical Engineering
Publication ISSN: 1552-4981
Last Modified: 02 Jul 2024 07:08
Date Deposited: 06 Dec 2019 15:00
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://onlinel ... 002/jbm.b.34486 (Publisher URL)
PURE Output Type: Article
Published Date: 2020-05
Published Online Date: 2019-10-04
Accepted Date: 2019-08-29
Authors: Lopes, João H.
Souza, Lucas P. (ORCID Profile 0000-0002-0188-5168)
Domingues, Juliana A.
Ferreira, Filipe V.
de Alencar Hausen, Moema
Camilli, José A.
Martin, Richard A. (ORCID Profile 0000-0002-6013-2334)
de Rezende Duek, Eliana A.
Mazali, Italo O.
Bertran, Celso A.



Version: Accepted Version

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