Evaluation of effectiveness of 45S5 bioglass doped with niobium for repairing critical-sized bone defect in in vitro and in vivo models


Here, we investigated the biocompatibility of a bioactive sodium calcium silicate glass containing 2.6 mol% Nb2O5 (denoted BGPN2.6) and compare the results with the archetypal 45S5 bioglass. The glass bioactivity was tested using a range of in vitro and in vivo experiments to assess its suitability for bone regeneration applications. in vitro studies consisted of assessing the cytocompatibility of the BGPN2.6 glass with bone-marrow-derived mesenchymal stem cells (BM-MSCs). Systemic biocompatibility was verified by means of the quantification of biochemical markers and histopathology of liver, kidneys, and muscles. The glass genotoxicity was assessed using the micronucleus test. The regeneration of a calvarial defect was assessed using both qualitative and quantitative analysis of three-dimensional microcomputed tomography images. The BGPN2.6 glass was not cytotoxic to BM-MSCs. It is systemically biocompatible causing no signs of damage to high metabolic and excretory organs such as the liver and kidneys. No mutagenic potential was observed in the micronucleus test. MicroCT images showed that BGPN2.6 was able to nearly fully regenerate a critical-sized calvarial defect and was far superior to standard 45S5 Bioglass. Defects filled with BGPN2.6 glass showed over 90% coverage compare to just 66% for 45S5 Bioglass. For one animal the defect was completely filled in 8 weeks. These results clearly show that Nb-containing bioactive glasses are a safe and effective biomaterial for bone replacement.

Publication DOI: https://doi.org/10.1002/jbm.a.36826
Divisions: College of Engineering & Physical Sciences > Aston Institute of Materials Research (AIMR)
College of Engineering & Physical Sciences
Funding Information: The authors acknowledge the use of the analytical instrumentation facility at Institute of Chemistry, University of Campinas, which is supported by the State of Paulo. This work was carried out with the support of the São Paulo Research Foundation – FAPES
Additional Information: This is the peer reviewed version of the following article: de Souza, LPL, Lopes, JH, Ferreira, FV, Martin, RA, Bertran, CA, Camilli, JA. Evaluation of effectiveness of 45S5 bioglass doped with niobium for repairing critical‐sized bone defect in in vitro and in vivo models. J Biomed Mater Res. 2019; 1– 12, which has been published in final form at https://doi.org/10.1002/jbm.a.36826.  This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Uncontrolled Keywords: bioactive glass,bone regeneration,genotoxicity,in vivo,niobium,Ceramics and Composites,Biomaterials,Biomedical Engineering,Metals and Alloys
Publication ISSN: 1552-4965
Last Modified: 02 Jul 2024 07:08
Date Deposited: 06 Dec 2019 14:53
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://onlinel ... 002/jbm.a.36826 (Publisher URL)
PURE Output Type: Article
Published Date: 2020-03
Published Online Date: 2019-11-07
Accepted Date: 2019-10-14
Authors: de Souza, Lucas P.L. (ORCID Profile 0000-0002-0188-5168)
Lopes, João H.
Ferreira, Filipe V.
Martin, Richard A. (ORCID Profile 0000-0002-6013-2334)
Bertran, Celso A.
Camilli, José A.



Version: Accepted Version

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