A mass spectrometry approach for the identification and localization of small aldehyde modifications of proteins


Lipids containing polyunsaturated fatty acids are primary targets of oxidation, which produces reactive short-chain aldehydes that can covalently modify proteins, a process called lipoxidation. Improved mass spectrometry (MS) methods for the analysis of these adducts in complex biological systems are needed. Lysozyme and human serum albumin (HSA) were used as model proteins to investigate lipoxidation products formed by two short-chain aldehydes, acrolein and pentanal, which are unsaturated and saturated aldehydes respectively. The adducts formed were stabilized by NaBH4 or NaBH3CN reduction and analysed by MS. Analysis of intact modified lysozyme showed a pentanal modification resulting from Schiff's base formation (+70 Da), and up to 8 acrolein adducts, all resulting from Michael addition (+58 Da). Analysis of tryptic digests identified specific histidine, cysteine and lysine residues modified in both lysozyme and HSA, and determined characteristic amino acid-specific fragmentations. Eight different internal fragment ions were found that could be used as general diagnostic ions for pentanal- and acrolein-modified amino acids. The combined use of intact protein analysis and LC-MS/MS methods provided a powerful tool for the identification and localization of aldehyde-protein adduct, and the diagnostic ions will facilitate the development of targeted MS methods for analysis of adducts in more complex samples.

Publication DOI: https://doi.org/10.1016/j.abb.2018.03.026
Dataset DOI: https://doi.org/10.17036/researchdata.aston.ac.uk.00000344
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > Aston Pharmacy School
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences > Chronic and Communicable Conditions
Additional Information: © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Funding: European Union's Horizon 2020 research and innovation programme under the Marie Sklowdowska-Curie grant agreement number 675132
Uncontrolled Keywords: Acrolein,Lipoxidation;,Pentanal,Diagnostic ions,Aldehydes,Immonium ion
Publication ISSN: 1096-0384
Last Modified: 08 Jul 2024 07:39
Date Deposited: 26 Mar 2018 07:40
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Related URLs: http://linkingh ... 003986117306720 (Publisher URL)
PURE Output Type: Article
Published Date: 2018-05-15
Published Online Date: 2018-03-23
Accepted Date: 2018-03-22
Authors: Afonso, Catarina B. (ORCID Profile 0000-0003-4828-7146)
Sousa, Bebiana C. (ORCID Profile 0000-0002-1356-6789)
Pitt, Andrew R. (ORCID Profile 0000-0003-3619-6503)
Spickett, Corinne M. (ORCID Profile 0000-0003-4054-9279)

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