Analytical Procedures for the Simultaneous Detection of Platinum Analogues

Abstract

Procedures for the analysis of two platinum analogues, Cisplatin and Carboplatin in aqueous solution, were reviewed and modified to produce a method to allow for the simultaneous detection of these two compounds, using high - performance liquid chromatography (HPLC). The HPLC assay method was used to investigate the decay of both platinum analogues in aqueous and biological samples. In addition the formation of the primary degredation product of Cisplatin, which was identified as the mono-aqua platinate II ion, was monitored. The decay was assessed by one of two methods of detection, using either an Electrochemical detector (EC), known for high sensitivity levels, or the severn Analytical UV detector. Stability studies of hydrolysis of Cisplatin have been well documented , but information as to the decay of Carboplatin is seen to conflict. The decay of Carboplatin in various infusion fluids was investigated over the period of two weeks, in a range of temperature and lighting conditions. Elevation of temperature has a significant effect on decay, as well as light and the presence of high chloride ion levels in the infusions.