Browse by Aston Author

2020

Olughu, Williams, Nienow, Alvin, Hewitt, Chris and Rielly, Chris (2020). Scale-down studies for the scale-up of a recombinant Corynebacterium glutamicum fed-batch fermentation:loss of homogeneity leads to lower levels of cadaverine production. Journal of Chemical Technology and Biotechnology, 95 (3), pp. 675-685.

2019

Moutsatsou, P., Ochs, J., Schmitt, R. H., Hewitt, C. J. and Hanga, M. P. (2019). Automation in cell and gene therapy manufacturing:from past to future. Biotechnology Letters, 41 (11), pp. 1245-1253.

Costariol, Elena, Rotondi, Marco, Amini, Arman, Hewitt, Christopher, Nienow, Alvin, Heathman, Thomas R.J., Micheletti, Martina and Rafiq, Qasim (2019). Establishing the scalable manufacture of primary human T-cells in an automated stirred-tank bioreactor. Biotechnology and Bioengineering, 116 (10), pp. 2488-2502.

Olughu, Williams, Deepika, Gurjot, Hewitt, Christopher and Rielly, Chris (2019). Insight into the large-scale upstream fermentation environment using scaled-down models. Journal of Chemical Technology and Biotechnology, 94 (3), pp. 647-657.

Heathman, Thomas R.j., Nienow, Alvin W., Rafiq, Qasim A., Coopman, Karen, Kara, Bo and Hewitt, Christopher J. (2019). Development of a process control strategy for the serum-free microcarrier expansion of human mesenchymal stem cells towards cost-effective and commercially viable manufacturing. Biochemical Engineering Journal, 141 , pp. 200-209.

2018

Heathman, Thomas R.j., Nienow, Alvin W., Rafiq, Qasim A., Coopman, Karen, Kara, Bo and Hewitt, Christopher J. (2018). Agitation and aeration of stirred-bioreactors for the microcarrier culture of human mesenchymal stem cells and potential implications for large-scale bioprocess development. Biochemical Engineering Journal, 136 , pp. 9-17.

Rafiq, Qasim A., Ruck, Steven, Hanga, Mariana P., Heathman, Thomas R.J., Coopman, Karen, Nienow, Alvin W., Williams, David J. and Hewitt, Christopher (2018). Qualitative and quantitative demonstration of bead-to-bead transfer with bone marrow-derived human mesenchymal stem cells on microcarriers:utilising the phenomenon to improve culture performance. Biochemical Engineering Journal, 135 , pp. 11-21.

2017

Rafiq, Qasim A., Hanga, Mariana P., Heathman, Thomas R.J., Coopman, Karen, Nienow, Alvin W., Williams, David J. and Hewitt, Christopher J. (2017). Process development of human multipotent stromal cell microcarrier culture using an automated high-throughput microbioreactor. Biotechnology and Bioengineering, 114 (10), pp. 2253-2266.

Murasiewicz, Halina, Nienow, Alvin W., Hanga, Mariana P., Coopman, Karen, Hewitt, Christopher J. and Pacek, Andrzej W. (2017). Engineering considerations on the use of liquid/liquid two-phase systems as a cell culture platform. Journal of Chemical Technology and Biotechnology, 92 (7), pp. 1690-1698.

Hanga, Mariana, Murasiewicz, Halina, Pacek, Andrzej W., Nienow, Alvin W., Coopman, Karen and Hewitt, Christopher (2017). Expansion of bone marrow-derived human mesenchymal stem/stromal cells (hMSCs) using a two-phase liquid/liquid system. Journal of Chemical Technology and Biotechnology, 92 (7), pp. 1577-1589.

2016

Nienow, A.W., Rafiq, Q.A., Heathman, T.R.J., Coopman, K. and Hewitt, C.J. (2016). Mixing theory for culture and harvest in bioreactors of human mesenchymal stem cells on microcarriers. Theoretical Foundations of Chemical Engineering, 50 (6), pp. 895-900.

Morris, Timothy J., Picken, Andrew, Sharp, Duncan M.C., Slater, Nigel K.H., Hewitt, Christopher J. and Coopman, Karen (2016). The effect of Me2SO overexposure during cryopreservation on HOS TE85 and hMSC viability, growth and quality. Cryobiology, 73 (3), pp. 367-375.

Hanga, Mariana P., Coopman, Karen, Nienow, Alvin W. and Hewitt, Christopher (2016). Perfluorocarbons:potential for successful expansion of bone marrow-derived human mesenchymal stem cells in a liquid/liquid two phase system. European Cells and Materials, 32 (Suppl.), p. 30.

Nienow, Alvin W., Hewitt, Christopher J., Heathman, Thomas R.J., Glyn, Veronica A.M., Fonte, Gonsalo N., Hanga, Mariana P., Coopman, Karen and Rafiq, Qasim A. (2016). Agitation conditions for the culture and detachment of hMSCs from microcarriers in multiple bioreactor platforms. Biochemical Engineering Journal, 108 , pp. 24-29.

Heathman, Thomas R.J., Rafiq, Qasim A., Chan, Alexander K.C., Coopman, Karen, Nienow, Alvin W., Kara, Bo and Hewitt, Christopher J. (2016). Characterization of human mesenchymal stem cells from multiple donors and the implications for large scale bioprocess development. Biochemical Engineering Journal, 108 , pp. 14-23.

Heathman, Thomas R.J., Stolzing, Alexandra, Fabian, Claire, Rafiq, Qasim A., Coopman, Karen, Nienow, Alvin W., Kara, Bo and Hewitt, Christopher J. (2016). Scalability and process transfer of mesenchymal stromal cell production from monolayer to microcarrier culture using human platelet lysate. Cytotherapy, 18 (4), pp. 523-535.

Rafiq, Qasim A., Coopman, Karen, Nienow, Alvin W. and Hewitt, Christopher J. (2016). Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors. Biotechnology Journal, 11 (4), 473–486.

2015

Heathman, Thomas R.J., Stolzing, Alexandra, Fabian, Claire, Rafiq, Qasim A., Coopman, Karen, Nienow, Alvin W., Kara, Bo and Hewitt, Christopher J. (2015). Serum-free process development:improving the yield and consistency of human mesenchymal stromal cell production. Cytotherapy, 17 (11), pp. 1524-1535.

Rafiq, Qasim A. and Hewitt, Christopher J. (2015). Cell therapies:why scale matters. Pharmaceutical Bioprocessing, 3 (2), pp. 97-99.

Heathman, Thomas R.J., Glyn, Veronica A.M., Picken, Andrew, Rafiq, Qasim A., Coopman, Karen, Nienow, Alvin W., Kara, Bo and Hewitt, Christopher J. (2015). Expansion, harvest and cryopreservation of human mesenchymal stem cells in a serum-free microcarrier process. Biotechnology and Bioengineering, 112 (8), pp. 1696-1707.

Heathman, Thomas R.J., Nienow, Alvin W., McCall, Mark J., Coopman, Karen, Kara, Bo and Hewitt, Christopher J. (2015). The translation of cell-based therapies:clinical landscape and manufacturing challenges. Regenerative medicine, 10 (1), pp. 49-64.

2014

Nienow, Alvin W., Rafiq, Qasim A., Coopman, Karen and Hewitt, Christopher J. (2014). A potentially scalable method for the harvesting of hMSCs from microcarriers. Biochemical Engineering Journal, 85 , pp. 79-88.

2011

Routledge, Sarah J., Hewitt, Christopher J., Bora, Nagamani and Bill, Roslyn M. (2011). Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield. Microbial Cell Factories, 10 (17),