Domain and nucleotide dependence of the interaction between Saccharomyces cerevisiae translation elongation factors 3 and 1A


Eukaryotic translation elongation factor 3 (eEF3) is a fungal-specific ATPase proposed to catalyze the release of deacylated-tRNA from the ribosomal E-site. In addition, it has been shown to interact with the aminoacyl-tRNA binding GTPase elongation factor 1A (eEF1A), perhaps linking the E and A sites. Domain mapping demonstrates that amino acids 775-980 contain the eEF1A binding sites. Domain III of eEF1A, which is also involved in actin-related functions, is the site of eEF3 binding. The binding of eEF3 to eEF1A is enhanced by ADP, indicating the interaction is favored post-ATP hydrolysis but is not dependent on the eEF1A-bound nucleotide. A temperature-sensitive P915L mutant in the eEF1A binding site of eEF3 has reduced ATPase activity and affinity for eEF1A. These results support the model that upon ATP hydrolysis, eEF3 interacts with eEF1A to help catalyze the delivery of aminoacyl-tRNA at the A-site of the ribosome. The dynamics of when eEF3 interacts with eEF1A may be part of the signal for transition of the post to pre-translocational ribosomal state in yeast.

Publication DOI:
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
Additional Information: © The American Society for Biochemistry and Molecular Biology, Inc.
Uncontrolled Keywords: eukaryotic translation elongation factor 3,deacylated-tRNA,E-site,aminoacyl-tRNA binding GTPase elongation factor 1A,eEF1A,A sites,amino acids 775-980,eEF3 binding,ADP,post-ATP hydrolysis,eEF1A-bound nucleotide,P915L,ATPase activity,ATP hydrolysis,aminoacyl-tRNA,translocational ribosomal state,yeast
Publication ISSN: 1083-351X
Last Modified: 27 Jun 2024 07:43
Date Deposited: 08 Sep 2010 10:40
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Related URLs: http://www.jbc. ... nt/281/43/32318 (Publisher URL)
PURE Output Type: Article
Published Date: 2006-10-27
Authors: Anand, Monika
Balar, Barvhi
Ulloque, Rory
Gross, Stephane R. (ORCID Profile 0000-0002-0867-8866)
Kinzy, Terri G.



Version: Published Version

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