Nuclear translocation of cytochrome c during apoptosis


Release of cytochrome c from mitochondria is a major event during apoptosis. Released cytochrome c has been shown to activate caspase-dependent apoptotic signals. In this report, we provide evidence for a novel role of cytochrome c in caspase-independent nuclear apoptosis. We showed that cytochrome c, released from mitochondria upon apoptosis induction, gradually accumulates in the nucleus as evidenced by both immunofluorescence and subcellular fractionation. Parallel to nuclear accumulation of cytochrome c, acetylated histone H2A, but not unmodified H2A, was released from the nucleus to the cytoplasm. Addition of purified cytochrome c to isolated nuclei recapitulated the preferential release of acetylated, but not deacetylated, histone H2A. Cytochrome c was also found to induce chromatin condensation. These results suggest that the nuclear accumulation of cytochrome c may be directly involved in the remodeling of chromatin. Our results provide evidence of a novel role for cytochrome c in inducing nuclear apoptosis.

Publication DOI:
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
Additional Information: © 2004 by The American Society for Biochemistry and Molecular Biology, Inc. Publisher's version/PDF may be used after a 12 months embargo period
Uncontrolled Keywords: cytochrome c,mitochondria,apoptosis,caspase-dependent,apoptotic signals,nuclear apoptosis
Publication ISSN: 1083-351X
Last Modified: 17 Jun 2024 07:08
Date Deposited: 15 Apr 2010 11:07
Full Text Link:
Related URLs: http://www.jbc. ... 9/24/24911.full (Publisher URL)
PURE Output Type: Article
Published Date: 2004-06-11
Authors: Nur-E-Kamal, Alam
Gross, Stephane R (ORCID Profile 0000-0002-0867-8866)
Pan, Zui
Balklava, Zita (ORCID Profile 0000-0001-9039-9710)
Ma, Jianjie
Liu, Leroy F



Version: Published Version

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