Poly(l-proline)-Stabilized Polypeptide Nanostructures via Ring-Opening Polymerization-Induced Self-Assembly (ROPISA)

Abstract

Poly(proline) II helical motifs located at the protein–water interface stabilize the three-dimensional structures of natural proteins. Reported here is the first example of synthetic biomimetic poly(proline)-stabilized polypeptide nanostructures obtained by a straightforward ring-opening polymerization-induced self-assembly (ROPISA) process through consecutive N-carboxyanhydride (NCA) polymerization. It was found that the use of multifunctional 8-arm initiators is critical for the formation of nanoparticles. Worm-like micelles as well as spherical morphologies were obtained as confirmed by dynamic light scattering (DLS), transmission electron microscopy (TEM), and small angle X-ray scattering (SAXS). The loading of the nanostructures with dyes is demonstrated. This fast and open-vessel procedure gives access to amino acids-based nanomaterials with potential for applications in nanomedicine.

Publication DOI: https://doi.org/10.1021/acsmacrolett.4c00400
Divisions: College of Engineering & Physical Sciences > Aston Institute of Materials Research (AIMR)
College of Engineering & Physical Sciences > Aston Advanced Materials
College of Engineering & Physical Sciences > Engineering for Health
College of Engineering & Physical Sciences > Aston Polymer Research Group
College of Engineering & Physical Sciences
College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry
Funding Information: E.T.-D. acknowledges funding through the postdoctoral grant Margarita Salas awarded in 2021 by Spanish Ministry of Universities. This project has received funding from the European Union\u2019s Horizon 2020 research and innovation program under the Marie
Additional Information: Copyright © 2024 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/).
Publication ISSN: 2161-1653
Data Access Statement: The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsmacrolett.4c00400.
Last Modified: 07 Oct 2024 07:59
Date Deposited: 08 Aug 2024 14:53
Full Text Link:
Related URLs: https://pubs.ac ... crolett.4c00400 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2024-08-20
Published Online Date: 2024-07-29
Accepted Date: 2024-07-23
Authors: Tinajero-Díaz, Ernesto
Judge, Nicola
Li, Bo
Leigh, Thomas
Murphy, Robert D.
Topham, Paul D. (ORCID Profile 0000-0003-4152-6976)
Derry, Matthew J. (ORCID Profile 0000-0001-5010-6725)
Heise, Andreas

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