Beyond life: Exploring hemodynamic patterns in postmortem mice brains

Abstract

We utilize Laser Speckle Contrast Imaging (LSCI) for visualizing cerebral blood flow in mice during and post-cardiac arrest. Analyzing LSCI images, we noted temporal blood flow variations across the brain surface for hours postmortem. Fast Fourier Transform (FFT) analysis depicted blood flow and microcirculation decay post-death. Continuous Wavelet Transform (CWT) identified potential cerebral hemodynamic synchronization patterns. Additionally, non-negative matrix factorization (NMF) with four components segmented LSCI images, revealing structural subcomponent alterations over time. This integrated approach of LSCI, FFT, CWT, and NMF offers a comprehensive tool for studying cerebral blood flow dynamics, metaphorically capturing the ‘end of the tunnel’ experience. Results showed primary postmortem hemodynamic activity in the olfactory bulbs, followed by blood microflow relocations between somatosensory and visual cortical regions via the superior sagittal sinus. This method opens new avenues for exploring these phenomena, potentially linking neuroscientific insights with mysteries surrounding consciousness and perception at life's end.

Publication DOI: https://doi.org/10.1002/jbio.202400017
Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
College of Health & Life Sciences
College of Engineering & Physical Sciences
Aston University (General)
Funding Information: Current study supported by COST Action CA21159 - Understanding interaction light - biological surfaces: possibility for new electronic materials and devices (PhoBioS), the European Union's Horizon 2020 research and innovation programme under grant agreeme
Additional Information: Copyright © 2024 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords: blood flow,brain microcirculation,continuous wavelet transform,laser speckle contrast,non-negative matrix factorization,postmortem
Publication ISSN: 1864-0648
Data Access Statement: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Last Modified: 03 Dec 2024 08:22
Date Deposited: 08 May 2024 14:00
Full Text Link:
Related URLs: https://onlinel ... /jbio.202400017 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2024-07
Published Online Date: 2024-05-07
Accepted Date: 2024-04-09
Authors: Sdobnov, Anton
Tsytsarev, Vassiliy
Piavchenko, Gennadi
Bykov, Alexander
Meglinski, Igor (ORCID Profile 0000-0002-7613-8191)

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