Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates


Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava (Manhiot esculenta) waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97% (w/w) using 3 M H2SO4, 120 min and 90 ºC. The ability of Cupriavidus necator to grow on CP hydrolysate and produce PHA was screened resulting in up to OD600 15.8 and 1.5 g/L of PHA (31% (gPHA/gDCW)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.

Publication DOI:
Divisions: College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry
College of Engineering & Physical Sciences
College of Engineering & Physical Sciences > Energy and Bioproducts Research Institute (EBRI)
Additional Information: This is an open access article distributed under the terms of the Creative Commons Attribution License CC BY [], which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords: Acid hydrolysis,Cassava,Cupriavidus necator,Polyhydroxyalkanoates,Waste valorization,Chemical Engineering (miscellaneous),Pollution,Waste Management and Disposal,Process Chemistry and Technology
Publication ISSN: 2213-3437
Last Modified: 21 Jun 2024 07:30
Date Deposited: 03 Jan 2024 13:06
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Related URLs: https://www.sci ... 21334372302554X (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2024-02
Published Online Date: 2023-12-25
Accepted Date: 2023-12-23
Authors: Hierro-Iglesias, Carmen
Fatokun, Cornelius O.
Chimphango, Annie
Bayitse, Richard
Blanco Sanchez, Paula Helena (ORCID Profile 0000-0002-9456-758X)
Thornley, Patricia (ORCID Profile 0000-0003-0783-2179)
Fernandez-Castane, Alfred (ORCID Profile 0000-0002-2572-7797)



Version: Accepted Version

License: Creative Commons Attribution

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