Hierro-Iglesias, Carmen, Fatokun, Cornelius O., Chimphango, Annie, Bayitse, Richard, Blanco Sanchez, Paula Helena, Thornley, Patricia and Fernandez-Castane, Alfred (2024). Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates. Journal of Environmental Chemical engineering, 12 (1),
Abstract
Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava (Manhiot esculenta) waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97% (w/w) using 3 M H2SO4, 120 min and 90 ºC. The ability of Cupriavidus necator to grow on CP hydrolysate and produce PHA was screened resulting in up to OD600 15.8 and 1.5 g/L of PHA (31% (gPHA/gDCW)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.
Publication DOI: | https://doi.org/10.1016/j.jece.2023.111815 |
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Divisions: | College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry College of Engineering & Physical Sciences College of Engineering & Physical Sciences > Energy and Bioproducts Research Institute (EBRI) Aston University (General) |
Additional Information: | This is an open access article distributed under the terms of the Creative Commons Attribution License CC BY [https://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Uncontrolled Keywords: | Acid hydrolysis,Cassava,Cupriavidus necator,Polyhydroxyalkanoates,Waste valorization,Chemical Engineering (miscellaneous),Pollution,Waste Management and Disposal,Process Chemistry and Technology |
Publication ISSN: | 2213-3437 |
Last Modified: | 04 Nov 2024 17:22 |
Date Deposited: | 03 Jan 2024 13:06 |
Full Text Link: | |
Related URLs: |
https://www.sci ... 21334372302554X
(Publisher URL) http://www.scop ... tnerID=8YFLogxK (Scopus URL) |
PURE Output Type: | Article |
Published Date: | 2024-02 |
Published Online Date: | 2023-12-25 |
Accepted Date: | 2023-12-23 |
Authors: |
Hierro-Iglesias, Carmen
Fatokun, Cornelius O. Chimphango, Annie Bayitse, Richard Blanco Sanchez, Paula Helena ( 0000-0002-9456-758X) Thornley, Patricia ( 0000-0003-0783-2179) Fernandez-Castane, Alfred ( 0000-0002-2572-7797) |