Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans

Abstract

Current medicine might be greatly enhanced by the ability to in vivo control and monitor neurons using opsins/phytochromes expressed in neural cells. The fundamental challenge with non-invasive neural cell activity regulation is a high absorption of visible light into biological tissues. This drawback could be mitigated by the photoconversion of phytochromes in spectral ranges with higher tissue transparency. In this study, we first demonstrated two-photon Pr-Pfr conversion of monomeric phytochrome at 1.2 µm wavelength. We did a comparison of linear and nonlinear conversion of truncated DrBphP bacterial phytochromes. This work provides a structured understanding of the optical properties of the dimer and monomer of phytochrome as well as their potential for use in optogenetics.

Publication DOI: https://doi.org/10.1117/12.2666486
Divisions: College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT)
College of Engineering & Physical Sciences
Funding Information: This work has been supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No.863214 – NEUROPA project. D.I.G. acknowledges the Rank Prize for the Return to Research grant.
Additional Information: Funding Information: This work has been supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No.863214 – NEUROPA project. D.I.G. acknowledges the Rank Prize for the Return to Research grant. Copyright 2023 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Uncontrolled Keywords: Adimer,iRFP,Monomer,Nonlinear conversion,Optogenetics,Pfr state,Phytochrome,Pr state,Tunable ultra-short pulsed laser,Two-photon photoconversion,Electronic, Optical and Magnetic Materials,Condensed Matter Physics,Computer Science Applications,Applied Mathematics,Electrical and Electronic Engineering
Publication ISSN: 1996-756X
Last Modified: 12 Apr 2024 07:19
Date Deposited: 25 Oct 2023 07:15
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.spi ... 6486.full?SSO=1 (Publisher URL)
PURE Output Type: Conference article
Published Date: 2023-08-11
Accepted Date: 2023-06-01
Authors: Galiakhmetova, Diana I.
Koviarov, Aleksandr S. (ORCID Profile 0000-0002-2668-2367)
Dremin, Viktor V. (ORCID Profile 0000-0001-6974-3505)
Gorodetsky, Andrei A.
Maimaris, Marios
Stoliarov, Dmitrii A. (ORCID Profile 0000-0001-8635-2346)
Baloban, Mikhail
Verkhusha, Vladislav V.
Sokolovski, Sergei G. (ORCID Profile 0000-0001-7445-7204)
Rafailov, Edik U. (ORCID Profile 0000-0002-4152-0120)

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