Transcranial Photosensitizer-Free Laser Treatment of Glioblastoma in Rat Brain

Abstract

Over sixty years, laser technologies have undergone a technological revolution and become one of the main tools in biomedicine, particularly in neuroscience, neurodegenerative diseases and brain tumors. Glioblastoma is the most lethal form of brain cancer, with very limited treatment options and a poor prognosis. In this study on rats, we demonstrate that glioblastoma (GBM) growth can be suppressed by photosensitizer-free laser treatment (PS-free-LT) using a quantum-dot-based 1267 nm laser diode. This wavelength, highly absorbed by oxygen, is capable of turning triplet oxygen to singlet form. Applying 1267 nm laser irradiation for a 4 week course with a total dose of 12.7 kJ/cm2 firmly suppresses GBM growth and increases survival rate from 34% to 64%, presumably via LT-activated apoptosis, inhibition of the proliferation of tumor cells, a reduction in intracranial pressure and stimulation of the lymphatic drainage and clearing functions. PS-free-LT is a promising breakthrough technology in non- or minimally invasive therapy for superficial GBMs in infants as well as in adult patients with high photosensitivity or an allergic reaction to PSs.

Publication DOI: https://doi.org/10.3390/ijms241813696
Divisions: College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT)
Funding Information: This research was supported by the academic leadership program Priority 2030 proposed by the First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University). O.S.-G., I.F., A.S., N.N., I.B., A.T., A.D., M.Z.
Additional Information: Copyright © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative CommonsAttribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/). Funding: O.S.-G., I.F., A.S., N.N., I.B., A.T., A.D., M.Z., A.E., D.Z., V.A., A.D., M.M. and V.K. were supported by a grant (No. 23-75-30001) from the Russian Science Foundation; N.N., D.Z. and V.A. were supported by a grant (No. 21-75-10088) from the Russian Science Foundation; O.S.-G., I.F., A.S., N.N., I.B., A.T. and M.T. were supported by a grant from the Russian Ministry of Science and Higher Education (No. 075-15-2022-1094); D.B. was supported by NIH R21NS091600 and R01 NS112808. E.R. and S.S. gratefully acknowledge funding from the HE Pathfinder GlioLight project (number 101129705). All cytological experiments, microscopy in the light field and fluorescence modes and electron microscopic examinations were carried out using equipment from the research equipment center “SYMBIOSIS” within research project No. GR 121031100266-3 (IBPPM RAS).
Uncontrolled Keywords: glioblastoma,phototherapy,quantum-dot 1267 nm laser,NIR laser oxidative cell damaging,lymphatic system
Publication ISSN: 1422-0067
Last Modified: 27 May 2024 07:41
Date Deposited: 15 Sep 2023 09:50
Full Text Link:
Related URLs: https://www.mdp ... 067/24/18/13696 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2023-09-05
Published Online Date: 2023-09-05
Accepted Date: 2023-09-02
Authors: Semyachkina-Glushkovskaya, Oxana
Sokolovski, Sergey (ORCID Profile 0000-0001-7445-7204)
Fedosov, Ivan
Shirokov, Alexander
Navolokin, Nikita
Bucharskaya, Alla
Blokhina, Inna
Terskov, Andrey
Dubrovski, Alexander
Telnova, Valeria
Tzven, Anna
Tzoy, Maria
Evsukova, Arina
Zhlatogosrkaya, Daria
Adushkina, Viktoria
Dmitrenko, Alexander
Manzhaeva, Maria
Krupnova, Valeria
Noghero, Alessio
Bragin, Denis
Bragina, Olga
Borisova, Ekaterina
Kurths, Jürgen
Rafailov, Edik (ORCID Profile 0000-0002-4152-0120)

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