Influence of various colloidal surfactants on the stability of MS2 bacteriophage suspension. The charge distribution on the PCV2 virus surface

Abstract

To understand virus stability in aqueous solutions, the colloidal nanostructure and properties of a model virus, the MS2 bacteriophage, have been investigated by studying the effect of the addition of electrolytes and various colloidal surfactants to its water solution at physiological conditions. The charge of the virus particles influences their colloidal properties. It was found that the ζ-potential value is reduced from –35 mV to –10 mV in 0.01 M CaCl2 and 0.1 M NaCl solutions as well as at higher electrolytes concentrations, while the size of the MS2 aggregates was about 600 ÷ 900 nm with individual particles of size around 30 nm also recorded. The 2: 1 electrolyte causes destabilization of MS2 bacteriophage particles in an aqueous solution at a lower concentration. The addition of cationic, anionic, and non-ionic colloidal surfactants below and above critical micelle concentration to MS2 bacteriophage suspension caused the destabilization of MS2 particles. We also investigated the capsid's surface of another virus, PCV2, using dynamic light scattering and laser Doppler electrophoresis. The hydrodynamic diameter and the ζ-potential of PCV2 empty capsid were found to be equal to 22 ± 1 nm and −41 ± 4 mV (using Ohshima approximations). The electrostatic potential of the surface was measured using acid-base probes and found to be equal to −91 ± 3 and +14 ± 2 mV for positively and negatively charged probes respectively, which indicate the ‘mosaic’ way of the charge distribution on the surface, similar to MS2′s surface studied previously. Our data provide new information about the virus surface, the complex process of virus aggregation-disaggregation and virus capsid disassembly.

Publication DOI: https://doi.org/10.1016/j.molliq.2023.122644
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
College of Engineering & Physical Sciences > School of Computer Science and Digital Technologies > Applied Mathematics & Data Science
College of Engineering & Physical Sciences > Systems analytics research institute (SARI)
College of Engineering & Physical Sciences
College of Engineering & Physical Sciences > School of Computer Science and Digital Technologies
College of Engineering & Physical Sciences > Engineering for Health
Funding Information: N. M.-P. thanks the Ministry of Education and Science of Ukraine for financial support in the frame of project #0122U001485. A. L. and V. F. thank the Ministry of Education and Science of Ukraine for financial support in the frame of project #0120U101064.
Additional Information: © 2023 Elsevier B.V. All rights reserved.
Uncontrolled Keywords: Charge distribution on capsid surface,Hydrodynamic diameter,MS2 bacteriophage,PCV2 capsid,Surface electrostatic potential,Zeta-potential,Electronic, Optical and Magnetic Materials,Atomic and Molecular Physics, and Optics,Condensed Matter Physics,Spectroscopy,Physical and Theoretical Chemistry,Materials Chemistry
Publication ISSN: 1873-3166
Last Modified: 23 May 2024 07:27
Date Deposited: 21 Aug 2023 15:07
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.sci ... 167732223014484 (Publisher URL)
PURE Output Type: Article
Published Date: 2023-10-01
Published Online Date: 2023-07-22
Accepted Date: 2023-07-19
Authors: Vodolazkaya, Natalya
Laguta, Anna
Farafonov, Vladimir
Nikolskaya, Marina
Balklava, Zita (ORCID Profile 0000-0001-9039-9710)
Khayat, Reza
Stich, Michael
Mchedlov-Petrossyan, Nikolay
Nerukh, Dmitry (ORCID Profile 0000-0001-9005-9919)

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Access Restriction: Restricted to Repository staff only until 22 July 2024.

License: Creative Commons Attribution Non-commercial No Derivatives


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