Efficiency of direct photoinduced generation of singlet oxygen at different wavelengths, power density and exposure time of laser irradiation


This work investigates the influence of laser irradiation parameters (wavelength, power density and exposure time) on singlet oxygen (1O2) generation efficiency. Chemical trap (L-histidine) and fluorescent probe (Singlet Oxygen Sensor Green, SOSG) detection methods were used. Studies have been conducted for 1267, 1244, 1122 and 1064 nm laser wavelengths. 1267 nm had the highest efficiency of 1O2 generation, but 1064 nm demonstrated almost the same efficiency. We also observed that the 1244 nm wavelength can generate some amount of 1O2. It was demonstrated that laser exposure time can generate 1O2 more efficiently than an increase of power. Additionally, the SOSG fluorescence intensity measurements method for acute brain slices was studied. This allowed us to evaluate the approach's potential for in vivo detection of 1O2 concentrations.

Publication DOI: https://doi.org/10.1039/D3AN00587A
Divisions: College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT)
College of Engineering & Physical Sciences
Additional Information: Funding: The study was supported by the Russian Science Foundation under the project no. 22-75-10088. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Publication ISSN: 1364-5528
Last Modified: 20 Jun 2024 07:30
Date Deposited: 04 Jul 2023 08:37
Full Text Link:
Related URLs: https://pubs.rs ... 3/AN/D3AN00587A (Publisher URL)
PURE Output Type: Article
Published Date: 2023-06-27
Accepted Date: 2023-06-23
Authors: Makovik, Irina
Vinokurov, Andrey
Dunaev, Andrey
Rafailov, Edik (ORCID Profile 0000-0002-4152-0120)
Dremin, Viktor (ORCID Profile 0000-0001-6974-3505)



Version: Published Version

License: Creative Commons Attribution

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