Evaluation of cell disruption technologies on magnetosome chain length and aggregation behaviour from Magnetospirillum gryphiswaldense MSR-1

Abstract

Magnetosomes are biologically-derived magnetic nanoparticles (MNPs) naturally produced by magnetotactic bacteria (MTB). Due to their distinctive characteristics, such as narrow size distribution and high biocompatibility, magnetosomes represent an attractive alternative to existing commercially-available chemically-synthesized MNPs. However, to extract magnetosomes from the bacteria, a cell disruption step is required. In this study, a systematic comparison between three disruption techniques (enzymatic treatment, probe sonication and high-pressure homogenization) was carried out to study their effect on the chain length, integrity and aggregation state of magnetosomes isolated from Magnetospirillum gryphiswaldense MSR-1 cells. Experimental results revealed that all three methodologies show high cell disruption yields (>89%). Transmission electron microscopy (TEM), dynamic light scattering (DLS) and, for the first time, nano-flow cytometry (nFCM) were employed to characterize magnetosome preparations after purification. TEM and DLS showed that high-pressure homogenization resulted in optimal conservation of chain integrity, whereas enzymatic treatment caused higher chain cleavage. The data obtained suggest that nFCM is best suited to characterize single membrane-wrapped magnetosomes, which can be particularly useful for applications that require the use of individual magnetosomes. Magnetosomes were also successfully labelled (>90%) with the fluorescent CellMask™ Deep Red membrane stain and analysed by nFCM, demonstrating the promising capacity of this technique as a rapid analytical tool for magnetosome quality assurance. The results of this work contribute to the future development of a robust magnetosome production platform.

Publication DOI: https://doi.org/10.3389/fbioe.2023.1172457
Divisions: College of Engineering & Physical Sciences > Aston Institute of Materials Research (AIMR)
College of Engineering & Physical Sciences
College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry
College of Engineering & Physical Sciences > Energy and Bioproducts Research Institute (EBRI)
College of Engineering & Physical Sciences > Aston Polymer Research Group
College of Engineering & Physical Sciences > Engineering for Health
College of Engineering & Physical Sciences > Aston Advanced Materials
Additional Information: Copyright © 2023 Masó-Martínez, Fryer, Aubert, Peacock, Lees, Rance, Fay, Topham and Fernández-Castané. This is an openaccess article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms Funding: This work was supported by the Royal Society Research Grant RGS\R1\191377, BBSRC New Investigators Award Grant No. BB/V010603/1, BBSRC NIBBs BIV E3B Metals in Biology Grant No. BB/S009787/1, NanoPrime EPSRC grant EP/R025282/1 and the Energy Research Accelerator (ERA) grant from Innovate UK (project No. 160052). MM-M acknowledges Aston University for an EPSRC-DTP-funded PhD studentship.
Uncontrolled Keywords: biomanufacturing,magnetic nanoparticles,magnetotactic bacteria,nano-flow cytometry,process analytical technology,Bioengineering,Biotechnology,Biomedical Engineering,Histology
Publication ISSN: 2296-4185
Last Modified: 13 Jun 2024 07:31
Date Deposited: 05 May 2023 09:18
Full Text Link:
Related URLs: https://www.fro ... 23.1172457/full (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2023-05-04
Accepted Date: 2023-04-21
Authors: Masó-Martínez, Marta
Fryer, Benjamin
Aubert, Dimitri
Peacock, Benjamin
Lees, Rebecca
Rance, Graham A.
Fay, Michael W.
Topham, Paul D. (ORCID Profile 0000-0003-4152-6976)
Fernández-Castané, Alfred (ORCID Profile 0000-0002-2572-7797)

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