2D Excitation-Emission Fluorescence Mapping Analysis of Plant Food Pigments


Homogeneous dispersion of plant food pigments is indispensable to study their characteristic fluorescence features for non-destructive rapid monitoring of food systems. However, it is highly challenging to obtain such optical grade homogenized stable dispersion of various plant pigments in aqueous media for tracing their precise fluorescence signatures. Herein, we demonstrate a unique strategy to disperse various pigments, such as chlorophylls, carotenoids and phenolic compounds by the high-speed shear-force mixing of fresh green and red bell peppers (capsicum annuum) in an aqueous medium with followed centrifugation and filtration. An advanced FLuorescence Excitation-emission (FLE) mapping and optical absorption analysis from the optical grade aqueous bell peppers dispersion allow simultaneous probing of chlorophylls, phenolic compounds and carotenoids by their characteristic electronic transitions. The demonstrated sampling protocols and spectroscopic analysis will be highly beneficial to obtain advanced spectroscopic databases from different food materials for rapid food analysis and quality control.

Publication DOI: https://doi.org/10.1016/j.foodchem.2023.135875
Divisions: College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT)
College of Engineering & Physical Sciences
College of Engineering & Physical Sciences > School of Computer Science and Digital Technologies > Electronics & Computer Engineering
College of Engineering & Physical Sciences > School of Computer Science and Digital Technologies
Additional Information: Copyright © 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/). Funding Information: The authors (Raghavan Chinnambedu Murugesan and Alex Rozhin) acknowledge the support of the Marie Skłodowska-Curie Individual Fellowship (MOFUS, # 795356) and of the Wolfson Foundation. Raghavan Chinnambedu Murugesan, Mohammed Thofike Ahmed Choudhury, and Alex Rozhin acknowledge the support of the Enabling Technologies & Innovation Competences Challenge (ETICC) Project, partly funded by the European Regional Development Funds (ERDF).
Uncontrolled Keywords: Pigment,chlorophyll,phenolic compounds,carotenoids,absorption,fluorescence
Publication ISSN: 0308-8146
Last Modified: 04 Jul 2024 07:19
Date Deposited: 05 Apr 2023 15:45
Full Text Link:
Related URLs: https://www.sci ... 308814623004922 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2023-08-30
Published Online Date: 2023-03-06
Accepted Date: 2023-03-03
Authors: Chinnambedu Murugesan, Raghavan
Choudhury, Mohammed Thofike Ahmed
Rozhin, Alex (ORCID Profile 0000-0003-2271-8227)



Version: Published Version

License: Creative Commons Attribution

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