Kerslake, Rachel, Panfilov, Suzana, Mustafa, Nashrah, Hall, Marcia, Kyrou, Ioannis, Randeva, Harpal S, Karteris, Emmanouil and Godfrey, Richard (2022). Elevated Circulating Lactate Levels and Widespread Expression of Its Cognate Receptor, Hydroxycarboxylic Acid Receptor 1 (HCAR1), in Ovarian Cancer. Journal of Clinical Medicine, 12 (1),
Abstract
Augmented glycolysis in cancer cells is a process required for growth and development. The Warburg effect provides evidence of increased glycolysis and lactic acid fermentation in cancer cells. The lactate end-product of glycolysis is receiving growing traction for its role as a cell signalling molecule. Ovarian cancer (OvCa) is also characterised by altered glucose metabolism. We aim to explore circulating lactate levels in patients with high-grade serous OvCa (HGSOC) and to elucidate the expression of the lactate receptor hydroxycarboxylic acid receptor 1 (HCAR1) in OvCa. HCAR1 expression was detected in patient biopsy cores using immunohistochemistry, while lactate was measured from whole blood with a Biosen-C line clinic measuring system. We noted significantly elevated lactate levels in OvCa patients (4.3 ± 1.9 mmol/L) compared with healthy controls (1.4 ± 0.6 mmol/L; < 0.0001), with an AUC of 0.96. The HCAR1 gene is overexpressed in OvCa compared to healthy controls ( < 0.001). Using an OvCa tissue microarray (>75% expression in 100 patients), high protein expression was also recorded across all epithelial OvCa subtypes and ovarian normal adjacent tissue (NAT). Lactate monitoring is a simple, cost-efficient test that can offer point-of-care results. Our data suggest that the potential of circulating lactate as a screening biomarker in OvCa merits further research attention.
Publication DOI: | https://doi.org/10.3390/jcm12010217 |
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Divisions: | College of Health & Life Sciences > Aston Medical School |
Additional Information: | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/) |
Uncontrolled Keywords: | lactate,liquid biopsy,screening,HCAR1,high-grade serous ovarian cancer (HGSOC),ovarian cancer (OvCa),biomarker |
Publication ISSN: | 2077-0383 |
Last Modified: | 06 Aug 2024 09:40 |
Date Deposited: | 23 Jan 2023 13:57 |
Full Text Link: | |
Related URLs: |
https://www.mdp ... 7-0383/12/1/217
(Publisher URL) |
PURE Output Type: | Article |
Published Date: | 2022-12-27 |
Published Online Date: | 2022-12-27 |
Accepted Date: | 2022-12-20 |
Submitted Date: | 2022-11-03 |
Authors: |
Kerslake, Rachel
Panfilov, Suzana Mustafa, Nashrah Hall, Marcia Kyrou, Ioannis ( ![]() Randeva, Harpal S Karteris, Emmanouil Godfrey, Richard |