Extensive respiratory chain defects in inhibitory interneurones in patients with mitochondrial disease


Aims: Mitochondrial disorders are among the most frequently inherited cause of neurological disease and arise due to mutations in mitochondrial or nuclear DNA. Currently, we do not understand the specific involvement of certain brain regions or selective neuronal vulnerability in mitochondrial disease. Recent studies suggest γ-aminobutyric acid (GABA)-ergic interneurones are particularly susceptible to respiratory chain dysfunction. In this neuropathological study, we assess the impact of mitochondrial DNA defects on inhibitory interneurones in patients with mitochondrial disease. Methods: Histochemical, immunohistochemical and immunofluorescent assays were performed on post-mortem brain tissue from 10 patients and 10 age-matched control individuals. We applied a quantitative immunofluorescent method to interrogate complex I and IV protein expression in mitochondria within GABAergic interneurone populations in the frontal, temporal and occipital cortices. We also evaluated the density of inhibitory interneurones in serial sections to determine if cell loss was occurring. Results: We observed significant, global reductions in complex I expression within GABAergic interneurones in frontal, temporal and occipital cortices in the majority of patients. While complex IV expression is more variable, there is reduced expression in patients harbouring m.8344A>G point mutations and POLG mutations. In addition to the severe respiratory chain deficiencies observed in remaining interneurones, quantification of GABAergic cell density showed a dramatic reduction in cell density suggesting interneurone loss. Conclusions: We propose that the combined loss of interneurones and severe respiratory deficiency in remaining interneurones contributes to impaired neuronal network oscillations and could underlie development of neurological deficits, such as cognitive impairment and epilepsy, in mitochondrial disease.

Publication DOI: https://doi.org/10.1111/nan.12238
Divisions: College of Health & Life Sciences > Aston Pharmacy School
Additional Information: © 2015 The Authors. This is an open access article under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Funding Information: This work was supported by The Wellcome Trust (074454/Z/04/Z), UK NIHR Biomedical Research Centre for Ageing and Age-Related disease award to the Newcastle upon Tyne Hospitals NHS Foundation Trust and the UK NHS Specialist Commissioners which funds the ''Rare Mitochondrial Disorders of Adults and Children'' Diagnostic Service in Newcastle upon Tyne (http://www .mitochondrialncg.nhs.uk). Tissue for this study was provided by the Newcastle Brain Tissue Resource which is funded in part by a grant from the UK Medical Research Council (G0400074) and the MRC Edinburgh Sudden Death Brain Bank.
Uncontrolled Keywords: Cognition,Epilepsy,Interneurones,Mitochondrial DNA,Respiratory chain deficiency,Pathology and Forensic Medicine,Histology,Neurology,Clinical Neurology,Physiology (medical)
Publication ISSN: 1365-2990
Full Text Link:
Related URLs: https://onlinel ... .1111/nan.12238 (Publisher URL)
PURE Output Type: Article
Published Date: 2016-02
Published Online Date: 2015-05-30
Accepted Date: 2015-03-18
Authors: Lax, Nichola Z.
Grady, John
Laude, Alex
Chan, Felix (ORCID Profile 0000-0002-4867-9636)
Hepplewhite, Philippa D.
Gorman, Grainne
Whittaker, Roger G.
Ng, Yi
Cunningham, Mark O.
Turnbull, Doug M.



Version: Published Version

License: Creative Commons Attribution

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