Evaluation of serum and tissue levels of VAP-1 in colorectal cancer

Abstract

BACKGROUND: The endothelial adhesion molecule, vascular adhesion protein-1 (VAP-1, AOC3) promotes lymphocyte recruitment to tumours, although the contribution that VAP-1 makes to lymphocyte recruitment in human colorectal cancer (CRC) is unknown. VAP-1 exists in circulating soluble form (sVAP-1). A previous study demonstrated elevated sVAP-1 levels in CRC patients. The aim of this study was to confirm this finding and study the differences in tissue VAP-1 expression between CRC and healthy tissues. METHODS: sVAP-1 levels were measured in the serum of 31 patients with CRC and 31 age- and sex-matched controls. Tissue VAP-1 levels were measured by immunohistochemistry, quantitative real-time PCR and Western blotting. RESULTS: The mean sVAP-1 level ± SD was significantly lower in the CRC group compared with the control group (399 ± 138 ng/ml versus 510 ± 142 ng/ml, P = 0.003). Tissue VAP-1 protein and mRNA levels were significantly lower in CRC compared with normal colon tissue. VAP-1 immunostaining was practically absent from CRC. CONCLUSIONS: VAP-1 is downregulated in human CRC and although the molecular basis of this down regulation is not yet known, we suggest it may be part of a mechanism used by the tumour to prevent the recruitment of anti-tumour immune cells. Our data contradicts the findings of others with regard sVAP-1 levels in patients with CRC. Possible reasons for this are discussed.

Publication DOI: https://doi.org/10.1186/s12885-016-2183-7
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
Additional Information: © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Funding Information: This article presents independent research funded by the National Institute for Health Research (NIHR), Medical Research Council (MR/J009962/1) and the Bowel Disease Research Foundation (BDRF).
Uncontrolled Keywords: VAP-1,,AOC3,Colorectal Cancel,Tumor Markers
Publication ISSN: 1471-2407
Last Modified: 05 Feb 2024 09:14
Date Deposited: 10 Nov 2022 14:35
Full Text Link:
Related URLs: https://bmccanc ... 016-2183-7#Ack1 (Publisher URL)
PURE Output Type: Article
Published Date: 2016-02-24
Accepted Date: 2016-02-16
Authors: Ward, Stephen T
Weston, Christopher J
Shepherd, Emma Louise (ORCID Profile 0000-0002-1345-1746)
Hejmadi, Rahul
Ismail, Tariq
Adams, David H.

Download

[img]

Version: Published Version

License: Creative Commons Attribution

| Preview

Export / Share Citation


Statistics

Additional statistics for this record