Surface-Modified Polypyrrole-Coated PLCL and PLGA Nerve Guide Conduits Fabricated by 3D Printing and Electrospinning

Abstract

The efficiency of nerve guide conduits (NGCs) in repairing peripheral nerve injury is not high enough yet to be a substitute for autografts and is still insufficient for clinical use. To improve this efficiency, 3D electrospun scaffolds (3D/E) of poly(l-lactide-co-ε-caprolactone) (PLCL) and poly(l-lactide-co-glycolide) (PLGA) were designed and fabricated by the combination of 3D printing and electrospinning techniques, resulting in an ideal porous architecture for NGCs. Polypyrrole (PPy) was deposited on PLCL and PLGA scaffolds to enhance biocompatibility for nerve recovery. The designed pore architecture of these “PLCL-3D/E” and “PLGA-3D/E” scaffolds exhibited a combination of nano- and microscale structures. The mean pore size of PLCL-3D/E and PLGA-3D/E scaffolds were 289 ± 79 and 287 ± 95 nm, respectively, which meets the required pore size for NGCs. Furthermore, the addition of PPy on the surfaces of both PLCL-3D/E (PLCL-3D/E/PPy) and PLGA-3D/E (PLGA-3D/E/PPy) led to an increase in their hydrophilicity, conductivity, and noncytotoxicity compared to noncoated PPy scaffolds. Both PLCL-3D/E/PPy and PLGA-3D/E/PPy showed conductivity maintained at 12.40 ± 0.12 and 10.50 ± 0.08 Scm–1 for up to 15 and 9 weeks, respectively, which are adequate for the electroconduction of neuron cells. Notably, the PLGA-3D/E/PPy scaffold showed superior cytocompatibility when compared with PLCL-3D/E/PPy, as evident via the viability assay, proliferation, and attachment of L929 and SC cells. Furthermore, analysis of cell health through membrane leakage and apoptotic indices showed that the 3D/E/PPy scaffolds displayed significant decreases in membrane leakage and reductions in necrotic tissue. Our finding suggests that these 3D/E/PPy scaffolds have a favorable design architecture and biocompatibility with potential for use in peripheral nerve regeneration applications.

Publication DOI: https://doi.org/10.1021/acs.biomac.2c00626
Divisions: College of Engineering & Physical Sciences > Aston Institute of Materials Research (AIMR)
College of Engineering & Physical Sciences > Aston Institute of Urban Technology and the Environment (ASTUTE)
College of Engineering & Physical Sciences
College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry
College of Engineering & Physical Sciences > Energy and Bioproducts Research Institute (EBRI)
Additional Information: Copyright © 2022 American Chemical Society. This accepted manuscript version is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License https://creativecommons.org/licenses/by-nc-nd/4.0/. Access to the Version of Record for M. Namhongsa, D. Daranarong, M. Sriyai, R. Molloy, S. Ross, G.M. Ross, A. Tuantranont, J. Tocharus, S. Jiraporn, S. Sivasinprasasn, P.D. Topham, B. Tighe, and W. Punyodom, (2022). 'Surface-Modified Polypyrrole-Coated PLCL and PLGA Nerve Guide Conduits Fabricated by 3D Printing and Electrospinning'. Biomacromolecules, - can be found here: https://doi.org/10.1021/acs.biomac.2c00626 Funding Information: The authors are thankful to the Royal Golden Jubilee for a Ph.D. Program (RGJ) and National Research University Project (NRU) for financial support as well as the Program Management Unit for Human Resources & Institutional Development, Research and Innovation, Office of National Higher Education Science Research and Innovation Policy Council (NXPO) [grant number B16F640001]; Fundamental Fund 2022, Chiang Mai University; Center of Excellence in Materials Science and Technology, Chiang Mai University; and the National Electronics and Computer Technology Center (NECTEC), NSTDA. This project was also partially funded from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 871650 (MEDIPOL).
Uncontrolled Keywords: Materials Chemistry,Polymers and Plastics,Biomaterials,Bioengineering
Publication ISSN: 1526-4602
Full Text Link:
Related URLs: https://pubs.ac ... .biomac.2c00626 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2022-11-14
Published Online Date: 2022-09-28
Accepted Date: 2022-09-12
Authors: Namhongsa, Manasanan
Daranarong, Donraporn
Sriyai, Montira
Molloy, Robert
Ross, Sukunya
Ross, Gareth M.
Tuantranont, Adisorn
Tocharus, Jiraporn
Sivasinprasasn, Sivanan
Topham, Paul D. (ORCID Profile 0000-0003-4152-6976)
Tighe, Brian (ORCID Profile 0000-0001-9601-8501)
Punyodom, Winita

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Access Restriction: Restricted to Repository staff only until 28 September 2023.

License: Creative Commons Attribution Non-commercial No Derivatives


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