Hothersall, Joanne, Osgerby, Alexander, Godfrey, Rita E, Overton, Tim W, Busby, Stephen J W and Browning, Douglas F (2022). New Vectors for Urea-Inducible Recombinant Protein Production. New Biotechnology, 72 , pp. 89-96.
Abstract
We have developed a novel urea-inducible recombinant protein production system by exploiting the Proteus mirabilis urease ureR-ureD promoter region and the ureR AraC-family transcriptional regulator. Experiments using the expression of β-galactosidase and green fluorescent protein (GFP) showed that promoter activity is tightly regulated and that varying the concentration of urea can give up to 100-fold induction. Production of proteins of biopharmaceutical interest has been demonstrated, including human growth hormone (hGH), a single chain antibody fragment (scFv) against interleukin-1β and a potential Neisserial vaccine candidate (BamAENm). Expression levels can be fine-tuned by temperature and different urea concentrations, and can be induced with readily available garden fertilisers and even urine. As urea is an inexpensive, stable inducer, a urea-induced expression system has the potential to considerably reduce the costs of large-scale recombinant protein production.
Publication DOI: | https://doi.org/10.1016/j.nbt.2022.10.003 |
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Divisions: | College of Health & Life Sciences > School of Biosciences Aston University (General) |
Additional Information: | © 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/). Funding statement: J.H. was generously funded by an Industrial Biotechnology Catalyst (Innovate UK, BBSRC, EPSRC) (BB/M018261/1) to support the translation, development and commercialisation of innovative Industrial Biotechnology processes, and, by a BBSRC IAA Follow-on-Fund award (BBSRC IAA BB/S506709/1). D.F.B was supported by BBSRC grants BB/M018261/1 and BB/R017689/1. AO was funded by a BBSRC PhD studentship as part of the Midlands Integrative Biosciences Training Partnership (MIBTP). |
Uncontrolled Keywords: | Escherichia coli,Recombinant protein production,Biopharmaceuticals,Transcription regulation,ureR,Urea |
Publication ISSN: | 1876-4347 |
Last Modified: | 09 Dec 2024 08:51 |
Date Deposited: | 26 Oct 2022 15:16 |
Full Text Link: | |
Related URLs: |
https://www.sci ... 871678422000577
(Publisher URL) |
PURE Output Type: | Article |
Published Date: | 2022-12-25 |
Published Online Date: | 2022-10-20 |
Accepted Date: | 2022-10-19 |
Authors: |
Hothersall, Joanne
Osgerby, Alexander Godfrey, Rita E Overton, Tim W Busby, Stephen J W Browning, Douglas F ( 0000-0003-4672-3514) |