Structural changes of erythrocyte membrane revealed by 3D confocal optical profilometer

Abstract

We examined hematological changes influenced by the experimental hypervitaminosis A. The 3D confocal optical profilometer was applied for assessment of the erythrocytes’ membrane structural changes influenced by an overdose of vitamin A. The blood smears were evaluated in terms of alterations of geometrical and optical parameters of erythrocytes for two groups of animals: oil base and retinol palmitate (n=9 animals for each group). The results demonstrate that an overdose of retinol palmitate causes changes in the torus curvature and pallor of discocytes, their surface area and volume. The observed structural malformations of the shape of red blood cells become visible at the earlier pre-clinical stage of changes in animal state and behavior. With this in mind, the results of the study open a new area of research in the certain dysfunction diagnosis of red blood cells and have a great potential in the further development of new curative protocols.

Publication DOI: https://doi.org/10.1002/jbio.202200222
Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
College of Engineering & Physical Sciences
Additional Information: © 2022 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Funding Information: Gennadii A. Piavchenko and Igor Meglinski acknowledge funding from the Academy of Finland (projects 325097 and 351068). This work was funded by the Ministry of Science and Higher Education of the Russian Federation within the framework of State support for the creation and development of World‐Class Research Centers “Digital Biodesign and Personalized Healthcare” No. 075‐15‐2022‐304 and partially funded by the Leverhulme Trust and The Royal Society (Ref. no.: APX111232 APEX Awards 2021). Tatiana A. Lomanovskaya and Gennadii A. Piavchenko contributed equally to this work. The authors acknowledge Mrs. Tatiana Koryashkina for her artwork in this article.
Uncontrolled Keywords: 3D confocal optical profilometry,erythrocyte morphology,hypervitaminosis A,morphodensitometric traits,retinol palmitate,Chemistry(all),Materials Science(all),Biochemistry, Genetics and Molecular Biology(all),Engineering(all),Physics and Astronomy(all)
Publication ISSN: 1864-0648
Last Modified: 27 Mar 2024 08:21
Date Deposited: 31 Aug 2022 14:15
Full Text Link:
Related URLs: https://onlinel ... /jbio.202200222 (Publisher URL)
PURE Output Type: Article
Published Date: 2022-08-04
Published Online Date: 2022-08-04
Accepted Date: 2022-08-02
Authors: Lomanovskaya, Tatiana A.
Piavchenko, Gennadii A.
Soldatov, Vladislav
Venediktov, Artem A.
Kuzmin, Egor A.
Kartashkina, Natalia L.
Mukhamedova, Svetlana G.
Boronikhina, Tatiana V.
Markov, Aleksandr G.
Telyshev, Dmitry V.
Meglinski, Igor (ORCID Profile 0000-0002-7613-8191)
Yatskovskiy, Alexander N.

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