Impact of Environmentally Relevant Concentrations of Bisphenol A (BPA) on the Gene Expression Profile in an In Vitro Model of the Normal Human Ovary


Endocrine-disrupting chemicals (EDCs), including the xenoestrogen Bisphenol A (BPA), can interfere with hormonal signalling. Despite increasing reports of adverse health effects associated with exposure to EDCs, there are limited data on the effect of BPA in normal human ovaries. In this paper, we present a detailed analysis of the transcriptomic landscape in normal Human Epithelial Ovarian Cells (HOSEpiC) treated with BPA (10 and 100 nM). Gene expression profiles were determined using high-throughput RNA sequencing, followed by functional analyses using bioin-formatics tools. In total, 272 and 454 differentially expressed genes (DEGs) were identified in 10 and 100 nM BPA-treated HOSEpiCs, respectively, compared to untreated controls. Biological pathways included mRNA surveillance pathways, oocyte meiosis, cellular senescence, and transcriptional misregulation in cancer. BPA exposure has a considerable impact on 10 genes: ANAPC2, AURKA, CDK1, CCNA2, CCNB1, PLK1, BUB1, KIF22, PDE3B, and CCNB3, which are also associated with progesterone-mediated oocyte maturation pathways. Future studies should further explore the effects of BPA and its metabolites in the ovaries in health and disease, making use of validated in vitro and in vivo models to generate data that will address existing knowledge gaps in basic biology, hazard characterisation, and risk assessment associated with the use of xenoestrogens such as BPA.

Publication DOI:
Divisions: College of Health & Life Sciences > Aston Medical School
Additional Information: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/).
Uncontrolled Keywords: BPA,Bisphenol A,EDC,endocrine-disrupting chemicals,ovarian cancer,ovary,Catalysis,Molecular Biology,Spectroscopy,Computer Science Applications,Physical and Theoretical Chemistry,Organic Chemistry,Inorganic Chemistry
Publication ISSN: 1422-0067
Last Modified: 15 Jul 2024 17:26
Date Deposited: 13 Jun 2022 08:35
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Related URLs: https://www.mdp ... 0067/23/10/5334 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2022-05-10
Accepted Date: 2022-05-03
Submitted Date: 2022-03-28
Authors: Zahra, Aeman
Kerslake, Rachel
Kyrou, Ioannis (ORCID Profile 0000-0002-6997-3439)
Randeva, Harpal S
Sisu, Cristina
Karteris, Emmanouil



Version: Published Version

License: Creative Commons Attribution

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