Al-Jubair, Tamim, Steffen, Jonas Hyld, Missel, Julie Winkel, Kitchen, Philip, Salman, Mootaz M, Bill, Roslyn M, Gourdon, Pontus and Törnroth-Horsefield, Susanna (2022). Characterization of human aquaporin protein-protein interactions using microscale thermophoresis (MST). STAR protocols, 3 (2),
Abstract
Aquaporin water channels (AQPs) are membrane proteins that maintain cellular water homeostasis. The interactions between human AQPs and other proteins play crucial roles in AQP regulation by both gating and trafficking. Here, we describe a protocol for characterizing the interaction between a human AQP and a soluble interaction partner using microscale thermophoresis (MST). MST has the advantage of low sample consumption and high detergent compatibility enabling AQP protein-protein interaction investigation with a high level of control of components and environment. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020) and Roche et al. (2017).
| Publication DOI: | https://doi.org/10.1016/j.xpro.2022.101316 |
|---|---|
| Divisions: | College of Health & Life Sciences College of Health & Life Sciences > School of Biosciences College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine |
| Additional Information: | © 2022 The Authors. This is an open access article under the CC BY license 4.0 Funding Information: We acknowledge the Swedish Research Council (to S.T.-H. through 2013-05945 and 2019-06106); the Crafoord Foundation (to S.T.-H. through 20140811 and 20180916); the Magnus Bergvall Foundation (to S.T.-H. through 2015-01534); the Knut and Alice Wallenberg Foundation (to P.G. through KAW 2015.0131 and 2020.0194); the Lundbeck Foundation (to P.G. through R133-A12689 and R313-2019-774); The Independent Research Fund Denmark (to P.G. through 4183-00559); NordForsk (to P.G. through 82000); UK Biotechnology & Biosciences Research Council (to R.M.B. and P.K. through BB/P025927/1); P.K. is the recipient of an Aston University 50th Anniversary Prize Fellowship: M.M.S. is supported through funding through funding by Leverhulme Trust (ECF-2021-602 ). |
| Uncontrolled Keywords: | Humans,Cell Membrane,Homeostasis,Single-molecule Assays,Proteins - metabolism,Aquaporins - metabolism,Protein Biochemistry,Proteins/metabolism,Aquaporins/metabolism,Medicine(all),Biochemistry, Genetics and Molecular Biology(all),Immunology and Microbiology(all),Neuroscience(all) |
| Publication ISSN: | 2666-1667 |
| Last Modified: | 15 Apr 2024 07:41 |
| Date Deposited: | 12 May 2022 08:15 |
| Full Text Link: | |
| Related URLs: |
https://www.sci ... 1964?via%3Dihub
(Publisher URL) http://www.scop ... tnerID=8YFLogxK (Scopus URL) |
PURE Output Type: | Article |
| Published Date: | 2022-06-17 |
| Published Online Date: | 2022-04-15 |
| Accepted Date: | 2022-04-01 |
| Authors: |
Al-Jubair, Tamim
Steffen, Jonas Hyld Missel, Julie Winkel Kitchen, Philip ( 
0000-0002-1558-4673)
Salman, Mootaz M Bill, Roslyn M (
0000-0003-1331-0852)
Gourdon, Pontus Törnroth-Horsefield, Susanna |
CORE (COnnecting REpositories)
CORE (COnnecting REpositories)