Study to investigate the prevalence of human papillomavirus in Barrett's oesophagus using a novel screening methodology


Introduction Human papillomavirus (HPV) is strongly associated with Barrett's dysplasia and oesophageal cancer suggesting a role in carcinogenesis. HPV persistence predicts treatment failure after endotherapy for Barrett's dysplasia. This pilot study applies a novel HPV screening tool (previously only used in the oropharynx) to detect HPV DNA directly and determine the prevalence rates in Barrett's oesophagus (BO). Method DNA was extracted from 20 formalin-fixed BO samples. HPV DNA was detected using real-time PCR and gel electrophoresis. Results 5 out of 20 patients were identified as positive for HPV. Prevalence was 25% in patients with BO. Conclusion This method can be used in BO's tissue to determine HPV infection. Adoption of this as a screening test could potentially revolutionise future research in this area. If a clear link between HPV and Barrett's dysplasia can be confirmed, this qPCR method has the potential to aid in monitoring and/or dysplasia detection by stratifying those most at risk and aid in the development of new therapies.

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Divisions: College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry
Additional Information: © Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See:
Uncontrolled Keywords: Barrett Esophagus - diagnosis - epidemiology,Barrett's oesophagus,polymerase chain reaction,Prevalence,Alphapapillomavirus,Humans,Papillomaviridae - genetics,Pilot Projects,oesophageal cancer,Papillomaviridae/genetics,Barrett Esophagus/diagnosis,Gastroenterology
Publication ISSN: 2054-4774
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Related URLs: https://bmjopen ... /1/ (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2022-04-04
Accepted Date: 2022-01-25
Submitted Date: 2021-11-15
Authors: White, Jonathan Richard
Ragunath, Krish
Whitton, Aimee
Marsh, Elizabeth
Kaye, Philip
Knight, Gillian (ORCID Profile 0000-0001-5334-2491)


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