A bilayered tissue engineered in vitro model simulating the tooth periodontium


Due to the complexity of the structure of the tooth periodontium, regeneration of the full tooth attachment is not a trivial task. There is also a gap in models that can represent human tooth attachment in vitro and in vivo. Aim of the study: to develop a bilayered in vitro construct that simulates the tooth periodontium, for the purpose of tissue regeneration and investigation of physiologic and orthodontic loading. Methods: Two types of materials were used to develop this construct: Sol-Gel 60S10Mg scaffold (University of Erlangen) representing the hard tissue component of the and commercially available Geistlich Bio-Gide® collagen membrane representing the soft tissue component of the tooth attachment. Each scaffold was dynamically seeded with periodontal ligament cells. The seeded scaffolds were either cultured separately, or combined in a bilayered construct, for 2 weeks. Characterization of the individual scaffolds and the bilayered constructs included biological characterization: (cell viability, SEM imaging to confirm cell attachment and viability qRT-PCR expression for periodontium regeneration markers), and mechanical characterisation of scaffolds and constructs. Results: HPDLCs enjoyed a biocompatible 3D environment within the bi-layered construct components as demonstrated by live/dead images and LDH assay. Gene expression showed variation between individual scaffolds and constructs detached from the bilayer. Most genes showed a drop in expression in the construct except for markers of angiogenesis which showed their highest expression in Bio-Gide® detached constructs. Conclusion and clinical significance: the development of this model is important for physiologic simulation invitro and for tissue regeneration purposes of the tooth periodontium.

Publication DOI: https://doi.org/10.22203/eCM.v042a17
Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
Additional Information: This article is distributed in accordance with Creative Commons Attribution Licence (http://creativecommons.org/licenses/by-sa/4.0/).
Uncontrolled Keywords: periodontal regeneration,physiologic simulation,in-vitro models,bilayered construct,tissue engineering,periodontal ligament cells,Bioengineering,Biochemistry,Biomaterials,Biomedical Engineering,Cell Biology
Publication ISSN: 1473-2262
Last Modified: 29 Apr 2024 07:37
Date Deposited: 07 Mar 2022 12:21
Full Text Link: https://www.ecm ... on/El-Gendy.pdf
Related URLs: https://www.ecm ... 2/vol042a17.php (Scopus URL)
https://www.ecm ... 2/vol042a17.php (Publisher URL)
PURE Output Type: Article
Published Date: 2021-10-11
Accepted Date: 2021-04-16
Authors: Khadre, Aliaa
Raif, El Mostafa
Junaid, S (ORCID Profile 0000-0001-9460-710X)
Goudouri, Ourania-Menti
Refaat, W
Ramadan, A
El-Gendy, Reem



Version: Published Version

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