Grainger, David C, Aiba, Hirofumi, Hurd, Douglas, Browning, Douglas F and Busby, Stephen J W (2007). Transcription factor distribution in Escherichia coli:studies with FNR protein. Nucleic Acids Research, 35 (1), pp. 269-278.
Abstract
Using chromatin immunoprecipitation (ChIP) and high-density microarrays, we have measured the distribution of the global transcription regulator protein, FNR, across the entire Escherichia coli chromosome in exponentially growing cells. Sixty-three binding targets, each located at the 5' end of a gene, were identified. Some targets are adjacent to poorly transcribed genes where FNR has little impact on transcription. In stationary phase, the distribution of FNR was largely unchanged. Control experiments showed that, like FNR, the distribution of the nucleoid-associated protein, IHF, is little altered when cells enter stationary phase, whilst RNA polymerase undergoes a complete redistribution.
Publication DOI: | https://doi.org/10.1093/nar/gkl1023 |
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Divisions: | College of Health & Life Sciences > School of Biosciences |
Additional Information: | © 2006 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Uncontrolled Keywords: | Binding Sites,Chromatin Immunoprecipitation,Chromosomes, Bacterial,DNA, Bacterial/chemistry,Escherichia coli/genetics,Escherichia coli Proteins/analysis,Iron-Sulfur Proteins/analysis,Oligonucleotide Array Sequence Analysis,Regulatory Elements, Transcriptional,Sequence Analysis, DNA,Transcription Factors/analysis |
Publication ISSN: | 1362-4962 |
Last Modified: | 06 Aug 2024 09:21 |
Date Deposited: | 04 Mar 2022 15:08 |
Full Text Link: | |
Related URLs: |
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PURE Output Type: | Article |
Published Date: | 2007-01-01 |
Authors: |
Grainger, David C
Aiba, Hirofumi Hurd, Douglas Browning, Douglas F ( ![]() Busby, Stephen J W |