Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro

Abstract

Background: DNA double strand breaks can affect genome integrity potentially leading to cancer. RAD51-associated protein 1 (RAD51AP1), an accessory protein to RAD51, is critical for homologous recombination, a key DNA damage response pathway. Emerging studies indicate a novel role for RAD51AP1 in carcinogenesis. Here we provide additional insight into the role of RAD51AP1 in ovarian cancer (OvCa). Methods: Gene expression and patient phenotype data were obtained from TCGA and GTEX project consortia for bioinformatics analysis. Immunohistochemistry of OvCa tissue microarray was undertaken. Functional analyses were performed in a SKOV3 OvCa cell line with down-regulation of RAD51AP1 using siRNA. Results: RAD51AP1 is overexpressed at gene level in primary and recurrent OvCa compared to controls. At protein level, RAD51AP1 was up-regulated in low grade serous tumors compared to high grade OvCa. There was higher expression of RAD51AP1 in OvCa metastatic to lymph nodes compared to primary cancer samples. Gene enrichment analyses identified 12 differentially expressed genes (DEGs) related to OvCa, eight of which are also common in tissue from patients with type 2 diabetes mellitus (T2DM). Conclusions: RAD51AP1 is overexpressed in OvCa, Given the link between OvCa and T2DM, the eight-gene signature shows potential for predictive value.

Publication DOI: https://doi.org/10.3390/jpm12020201
Divisions: College of Health & Life Sciences > Aston Medical School
Additional Information: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Uncontrolled Keywords: ovarian cancer,RAD51AP1,biomarker,T2DM
Publication ISSN: 2075-4426
Last Modified: 04 Apr 2024 07:40
Date Deposited: 03 Feb 2022 08:46
Full Text Link:
Related URLs: https://www.mdp ... 5-4426/12/2/201 (Publisher URL)
PURE Output Type: Article
Published Date: 2022-02-01
Accepted Date: 2022-01-25
Authors: Filipe, Alice
Katopodis, Periklis
Chudasama, Dimple
Kerslake, Rachel
Jeyaneethi, Jeyarooban
Anikin, Vladimir
Silva, Elisabete
Kyrou, Ioannis (ORCID Profile 0000-0002-6997-3439)
Randeva, Harpal S.
Sisu, Cristina
Hall, Marcia
Karteris, Emmanouil

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