Kamenšek, Simona, Browning, Douglas F, Podlesek, Zdravko, Busby, Stephen J W, Žgur-Bertok, Darja and Butala, Matej (2015). Silencing of DNase Colicin E8 Gene Expression by a Complex Nucleoprotein Assembly Ensures Timely Colicin Induction. PLoS Genetics, 11 (6),
Abstract
Colicins are plasmid-encoded narrow spectrum antibiotics that are synthesized by strains of Escherichia coli and govern intraspecies competition. In a previous report, we demonstrated that the global transcriptional factor IscR, co dependently with the master regulator of the DNA damage response, LexA, delays induction of the pore forming colicin genes after SOS induction. Here we show that IscR is not involved in the regulation of nuclease colicins, but that the AsnC protein is. We report that AsnC, in concert with LexA, is the key controller of the temporal induction of the DNA degrading colicin E8 gene (cea8), after DNA damage. We demonstrate that a large AsnC nucleosome-like structure, in conjunction with two LexA molecules, prevent cea8 transcription initiation and that AsnC binding activity is directly modulated by L asparagine. We show that L-asparagine is an environmental factor that has a marked impact on cea8 promoter regulation. Our results show that AsnC also modulates the expression of several other DNase and RNase colicin genes but does not substantially affect pore-forming colicin K gene expression. We propose that selection pressure has "chosen" highly conserved regulators to control colicin expression in E. coli strains, enabling similar colicin gene silencing among bacteria upon exchange of colicinogenic plasmids.
Publication DOI: | https://doi.org/10.1371/journal.pgen.1005354 |
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Divisions: | College of Health & Life Sciences > School of Biosciences |
Additional Information: | © 2015 Kamenšek et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Funding: DFB was supported by BBSRC grant BB/J006076/1 (http://www.bbsrc.ac.uk/), awarded to both DFB and SJWB. SK was supported by grant from the Slovenian Research Agency (J3-3624) (https://www.arrs.gov.si/en/novo.asp). ZP and DŽB were supported by grant from the Slovenian Research Agency (P1-0198). MB was supported by grant from the Slovenian Research Agency (P1-0207). |
Uncontrolled Keywords: | Asparagine/metabolism,Bacterial Proteins/genetics,Colicins/genetics,Escherichia coli/genetics,Escherichia coli Proteins/genetics,Gene Expression Regulation, Bacterial,Nucleoproteins/genetics,Promoter Regions, Genetic,SOS Response, Genetics,Serine Endopeptidases/genetics,Trans-Activators/genetics,Transcription Factors/genetics |
Publication ISSN: | 1553-7404 |
Last Modified: | 06 Aug 2024 09:21 |
Date Deposited: | 18 Jan 2022 13:54 |
Full Text Link: | |
Related URLs: |
https://journal ... al.pgen.1005354
(Publisher URL) |
PURE Output Type: | Article |
Published Date: | 2015-06-26 |
Authors: |
Kamenšek, Simona
Browning, Douglas F ( ![]() Podlesek, Zdravko Busby, Stephen J W Žgur-Bertok, Darja Butala, Matej |