BamA and BamD Are Essential for the Secretion of Trimeric Autotransporter Adhesins

Abstract

The BAM complex in Escherichia coli is composed of five proteins, BamA-E. BamA and BamD are essential for cell viability and are required for the assembly of β-barrel outer membrane proteins. Consequently, BamA and BamD are indispensable for secretion via the classical autotransporter pathway (Type 5a secretion). In contrast, BamB, BamC, and BamE are not required for the biogenesis of classical autotransporters. Recently, we demonstrated that TamA, a homologue of BamA, and its partner protein TamB, were required for efficient secretion of proteins via the classical autotransporter pathway. The trimeric autotransporters are a subset of the Type 5-secreted proteins. Unlike the classical autotransporters, they are composed of three identical polypeptide chains which must be assembled together to allow secretion of their cognate passenger domains. In contrast to the classical autotransporters, the role of the Bam and Tam complex components in the biogenesis of the trimeric autotransporters has not been investigated fully. Here, using the Salmonella enterica trimeric autotransporter SadA and the structurally similar YadA protein of Yersinia spp., we identify the importance of BamA and BamD in the biogenesis of the trimeric autotransporters and reveal that BamB, BamC, BamE, TamA and TamB are not required for secretion of functional passenger domain on the cell surface. Importance: The secretion of trimeric autotransporters (TAA's) has yet to be fully understood. Here we show that efficient secretion of TAAs requires the BamA and D proteins, but does not require BamB, C or E. In contrast to classical autotransporter secretion, neither trimeric autotransporter tested required TamA or B proteins to be functionally secreted.

Publication DOI: https://doi.org/10.3389/fmicb.2021.628879
Divisions: College of Health & Life Sciences > School of Biosciences
Additional Information: © 2021 Rooke, Icke, Wells, Rossiter, Browning, Morris, Leo, Schütz, Autenrieth, Cunningham, Linke and Henderson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Publication ISSN: 1664-302X
Last Modified: 01 Apr 2024 07:41
Date Deposited: 13 Jan 2022 15:35
Full Text Link:
Related URLs: https://www.fro ... 021.628879/full (Publisher URL)
PURE Output Type: Article
Published Date: 2021-02-23
Accepted Date: 2021-01-26
Authors: Rooke, Jessica L
Icke, Christopher
Wells, Timothy J
Rossiter, Amanda E
Browning, Douglas F (ORCID Profile 0000-0003-4672-3514)
Morris, Faye C
Leo, Jack C
Schütz, Monika S
Autenrieth, Ingo B
Cunningham, Adam F
Linke, Dirk
Henderson, Ian R

Download

[img]

Version: Published Version

License: Creative Commons Attribution

| Preview

Export / Share Citation


Statistics

Additional statistics for this record