Screening of Alzheimer’s disease with multiwavelength Stokes polarimetry in a mouse model


The minimum histological criterion for the diagnostics of Alzheimer’s disease (AD) in tissue is the presence of senile plaques and neurofibrillary tangles in specific brain locations. The routine procedure of morphological analysis implies time-consuming and laborious steps including sectioning and staining of formalin-fixed paraffin-embedded (FFPE) tissue. We developed a multispectral Stokes polarimetric imaging approach that allows characterization of FFPE brain tissue samples to discern the stages of AD progression without sectioning and staining the tissue. The Stokes polarimetry approach is highly sensitive to structural alterations of brain tissue, particularly to the changes in light scattering and birefringence. We present the results of the label-free non-destructive screening of FFPE mouse brain tissue and show several polarization metrics that demonstrate statistically significant differences for tissues at different stages of AD.

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Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
College of Engineering & Physical Sciences
Additional Information: This work is licensed under a Creative Commons Attribution 4.0 License. For more information, see Funding: This work was supported by the Academy of Finland (grants 314369 and 325097), the ATTRACT project funded by the EC under Grant Agreement 777222, the INFOTECH strategic fund. The work of Mariia Borovkova was also supported by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No.713606. The work of Jens Pahnke was supported by the following grants: Deutsche Forschungsgemeinschaft/ Germany (DFG 263024513); Latvian Council of Science/ Latvia (ShortAbeta lzp-2018/1-0275); HelseSØ/ Norway (2019054, 2019055); EEA grant/Norges grants (TAČR TARIMAD TO100078); Norges forskningsrådet/ Norway (260786 PROPAD, 295910 NAPI, PETABC); European Commission (643417). PROP-AD and PETABC are EU Joint Programme - Neurodegenerative Disease Research (JPND) projects. PROP-AD is supported through the following funding organizations under the aegis of JPND - The research was also partially supported by a grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021) and the Ministry of Science and Higher Education of the Russian Federation within the framework of state support for the creation and development of World-Class Research Centers “Digital biodesign and personalized healthcare” № 075-15-2020-926
Uncontrolled Keywords: Ab plaques,Brain,Laser beams,Measurement by laser beam,Mice,Microscopy,Optical microscopy,Optical polarimetry,Polarimetry,Stokes vector,birefringence,scattering,Software,Radiological and Ultrasound Technology,Computer Science Applications,Electrical and Electronic Engineering
Publication ISSN: 1558-254X
Last Modified: 09 Apr 2024 17:50
Date Deposited: 25 Nov 2021 11:05
Full Text Link:
Related URLs: https://ieeexpl ... cument/9622320/ (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2021-11-22
Published Online Date: 2021-11-22
Accepted Date: 2021-11-01
Authors: Borovkova, Mariia
Sieryi, Oleksii
Lopushenko, Ivan
Kartashkina, Natalia
Pahnke, Jens
Bykov, Alexander
Meglinski, Igor (ORCID Profile 0000-0002-7613-8191)



Version: Accepted Version

License: Creative Commons Attribution

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