Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro


We evaluated whether insulin could stimulate β-alanine uptake by skeletal muscle cells in vitro. Mouse myoblasts (C2C12) (n = 3 wells per condition) were cultured with β-alanine (350 or 700 µmol·L−1), with insulin (100 µU·mL−1) either added to the media or not. Insulin stimulated the β-alanine uptake at the lower (350 µmol·L−1) but not higher (700 µmol·L−1) β-alanine concentration in culture medium, indicating that transporter saturation might blunt the stimulatory effects of insulin.

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Divisions: College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry
Additional Information: Funding Information: L.S.G: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); G.G.A: São Paulo Research Foundation—FAPESP (#2014/11948-8; 2019/25032-9). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES-Finance Code 001). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit
Uncontrolled Keywords: Carnosine,Insulin,Taurine transporter,β-alanine,Biochemistry,Organic Chemistry,Clinical Biochemistry
Publication ISSN: 1438-2199
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://link.sp ... 726-021-03090-9 (Publisher URL)
PURE Output Type: Article
Published Date: 2021-11
Published Online Date: 2021-10-21
Accepted Date: 2021-10-07
Authors: Santos, Lívia
Gonçalves, L. S.
Bagheri-Hanei, Shirin
Möller, Gabriella Berwig
Sale, Craig
James, Ruth M.
Artioli, Guilherme Giannini



Version: Published Version

License: Creative Commons Attribution

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