In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy

Abstract

The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation.

Publication DOI: https://doi.org/10.1117/12.2595118
Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
College of Engineering & Physical Sciences
Additional Information: Copyright 2021 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. Funding Information: This work was partially funded by the China Scholarship Council (CSC No. 201706410089, R.Z.). R.Z. acknowledges X.Y. Su (School of Science, Beijing Jiaotong University, Beijing, China) for technical help in laser irradiation source selection. The authors also acknowledge the contribution of Russian Science Foundation (Projects: 19-72-30012 and 20-64-46003). I.M. acknowledges partial support from the Academy of Finland (project 325,097).
Uncontrolled Keywords: laser irradiation,light microscopy,optical tweezers,RBC aggregation,Red blood cells (RBCs),Electronic, Optical and Magnetic Materials,Condensed Matter Physics,Computer Science Applications,Applied Mathematics,Electrical and Electronic Engineering
Publication ISSN: 1996-756X
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.spi ... 5118.full?SSO=1 (Publisher URL)
PURE Output Type: Conference article
Published Date: 2021-08-01
Accepted Date: 2021-08-01
Authors: Zhu, Ruixue
Avsievich, Tatiana
Popov, Alexey
Bykov, Alexander
Meglinski, Igor (ORCID Profile 0000-0002-7613-8191)

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