Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest

Abstract

The impairments of cerebral blood flow microcirculation brought on by cardiac and respiratory arrest were assessed with multi-modal diagnostic facilities, utilising laser speckle contrast imaging, fluorescence spectroscopy and diffuse reflectance spectroscopy. The results of laser speckle contrast imaging show a notable reduction of cerebral blood flow in small and medium size vessels during a few minutes of respiratory arrest, while the same effect was observed in large sinuses and their branches during the circulatory cessation. Concurrently, the redox ratio assessed with fluorescence spectroscopy indicates progressing hypoxia, NADH accumulation and increase of FAD consumption. The results of diffuse reflectance spectra measurements display a more rapid grow of the perfusion of deoxygenated blood in case of circulatory impairment. In addition, consequent histopathological analysis performed by using new tissue staining procedure developed in-house. It shows notably higher reduction of size of the neurons due to their wrinkling within brain tissues influenced by circulation impair. Whereas, the brain tissues altered with the respiratory arrest demonstrate focal perivascular oedema and mild hypoxic changes of neuronal morphology. Thus, the study suggests that consequences of a cessation of cerebral blood flow become more dramatic and dangerous compare to respiratory arrest.

Publication DOI: https://doi.org/10.1002/jbio.202100216
Divisions: College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT)
College of Engineering & Physical Sciences
College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
Additional Information: © 2021 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Funding: The authors disclose receipt of financial support for the research, authorship, and/or publication of this article as fol-lowing: G.P. and S.K. acknowledge the support from the Ministry of Science and Higher Education of the Russian Federation withing the framework of State support for the creation and development of World-Class research Centres “Digital Biodesign and Personalized Healthcare” No 075-15-2020-926.V.D. kindly acknowledges personal support from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 839888.A.D. and I.M. acknowledge funding from the Academy of Fin-land (grant No. 326204). This work has been also partially supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No.863214- NEUROPA project.
Uncontrolled Keywords: brain imaging,cardiac cessation,cerebral blood flow,laser speckle contrast,microcirculation,respiratory arrest,Chemistry(all),Materials Science(all),Biochemistry, Genetics and Molecular Biology(all),Engineering(all),Physics and Astronomy(all)
Publication ISSN: 1864-0648
Full Text Link:
Related URLs: https://onlinel ... /jbio.202100216 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2021-09-17
Published Online Date: 2021-09-17
Accepted Date: 2021-09-09
Authors: Piavchenko, Gennadii
Kozlov, Igor
Dremin, Viktor (ORCID Profile 0000-0001-6974-3505)
Stavtsev, Dmitry
Seryogina, Evgeniya
Kandurova, Ksenia
Shupletsov, Valery
Lapin, Konstantin
Alekseyev, Alexander
Kuznetsov, Sergey
Bykov, Alexander
Dunaev, Andrey
Meglinski, Igor (ORCID Profile 0000-0002-7613-8191)

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