Observation of arenavirus nucleoprotein heptamer assembly


Arenaviruses are enveloped viruses containing a segmented, negative, and ambisense single-stranded RNA genome wrapped with a nucleoprotein (NP). The NP is the most abundant viral protein in infected cells and plays a critical role in both replication/transcription and virion assembly. The NP associates with RNA to form a ribonucleoprotein (RNP) complex, and this implies self-assembly while the exact structure of this polymer is not yet known. Here, we report a measurement of the full-length Mopeia virus NP by negative stain transmission electron microscopy. We observed RNP complex particles with diameter 15 ± 1 nm as well as symmetric circular heptamers of the same diameter, consistent with previous observations.

Publication DOI: https://doi.org/10.1002/2211-5463.13106
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences
Additional Information: © 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Funding: Agence Nationale de la Recherche. Grant Numbers: FRISBI - ANR-10-INSB-05-01, PANUVI - ANR-18-ASTR-0010-01 Fondation Infection Méditerranée
Uncontrolled Keywords: General Biochemistry, Genetics and Molecular Biology
Publication ISSN: 2211-5463
Last Modified: 19 Mar 2024 08:32
Date Deposited: 17 Sep 2021 07:11
Full Text Link:
Related URLs: https://febs.on ... 2211-5463.13106 (Publisher URL)
PURE Output Type: Article
Published Date: 2021-04
Published Online Date: 2021-02-25
Accepted Date: 2021-02-01
Authors: Papageorgiou, Nicolas
Vaitsopoulou, Afroditi
Diop, Awa
Nguyen, Thi Hong Van
Canard, Bruno
Alvarez, Karine
Ferron, François



Version: Published Version

License: Creative Commons Attribution

| Preview

Export / Share Citation


Additional statistics for this record