Novel, Fully Characterised Bovine Taste Bud Cells of Fungiform Papillae

Abstract

Current understanding of functional characteristics and biochemical pathways in taste bud cells have been hindered due the lack of long-term cultured cells. To address this, we developed a holistic approach to fully characterise long term cultured bovine taste bud cells (BTBCs). Initially, cultured BTBCs were characterised using RT-PCR gene expression profiling, immunocytochemistry, flowcytometry and calcium imaging, that confirmed the cells were mature TBCs that express taste receptor genes, taste specific protein markers and capable of responding to taste stimuli, i.e., denatonium (2 mM) and quinine (462.30 μM). Gene expression analysis of forty-two genes implicated in taste transduction pathway (map04742) using custom-made RT-qPCR array revealed high and low expressed genes in BTBCs. Preliminary datamining and bioinformatics demonstrated that the bovine α-gustducin, gustatory G-protein, have higher sequence similarity to the human orthologue compared to rodents. Therefore, results from this work will replace animal experimentation and provide surrogate cell-based throughput system to study human taste transduction.

Publication DOI: https://doi.org/10.3390/cells10092285
Divisions: College of Health & Life Sciences > Aston Pharmacy School
College of Health & Life Sciences > Clinical and Systems Neuroscience
College of Health & Life Sciences
College of Health & Life Sciences > Chronic and Communicable Conditions
Additional Information: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Funding: This work was supported by grant from the National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) (NC/N003039/1).
Uncontrolled Keywords: taste bud cells,taste cell characterization,PCR
Full Text Link:
Related URLs: https://www.mdp ... -4409/10/9/2285 (Publisher URL)
PURE Output Type: Article
Published Date: 2021-09-02
Accepted Date: 2021-08-28
Authors: Ftuwi, Habtom
Parri, Rheinallt (ORCID Profile 0000-0002-1412-2688)
Mohammed, Afzal R. (ORCID Profile 0000-0002-5212-3040)

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