Mandair, Ravneet, Karagoz, Pinar and Bill, Roslyn M. (2021). A redox-neutral, two-enzyme cascade for the production of malate and gluconate from pyruvate and glucose. Applied Sciences, 11 (11),
Abstract
A triple mutant of NADP(H)-dependent malate dehydrogenase from thermotolerant Thermococcus kodakarensis has an altered cofactor preference for NAD+, as well as improved malate production compared to wildtype malate dehydrogenase. By combining mutant malate dehydrogenase with glucose dehydrogenase from Sulfolobus solfataricus and NAD+/NADH in a closed reaction environment, gluconate and malate could be produced from pyruvate and glucose. After 3 h, the yield of malate was 15.96 mM. These data demonstrate the feasibility of a closed system capable of cofactor regeneration in the production of platform chemicals.
| Publication DOI: | https://doi.org/10.3390/app11114877 |
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| Divisions: | College of Health & Life Sciences > School of Biosciences College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine College of Health & Life Sciences |
| Additional Information: | Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/. Funding: This research was funded by the Biotechnology and Biological Sciences Research Council (BBSRC) through the Global Challenges Research Fund Project, CAPRI-BIO (BB/P022685/1). R.M. was supported by a BBSRC training grant with Chemoxy Ltd. (BB/M016668/1). The APC was funded by BBSRC. |
| Uncontrolled Keywords: | Enzymatic cascade reaction,malate production,malate dehydrogenase,glucose dehydrogenase,Malate production,Glucose dehydrogenase,Malate dehydrogenase,Engineering(all),Instrumentation,Materials Science(all),Fluid Flow and Transfer Processes,Process Chemistry and Technology,Computer Science Applications |
| Publication ISSN: | 2076-3417 |
| Last Modified: | 23 Apr 2024 07:19 |
| Date Deposited: | 02 Jun 2021 13:32 |
| Full Text Link: | |
| Related URLs: |
https://www.mdp ... 3417/11/11/4877
(Publisher URL) http://www.scop ... tnerID=8YFLogxK (Scopus URL) |
PURE Output Type: | Article |
| Published Date: | 2021-05-26 |
| Accepted Date: | 2021-05-24 |
| Authors: |
Mandair, Ravneet
Karagoz, Pinar Bill, Roslyn M. ( 
0000-0003-1331-0852)
|
CORE (COnnecting REpositories)
CORE (COnnecting REpositories)