Fernandez-Castane, Alfred, Overton, Tim W., Li, Hong, Joseph, Stephan, Ebeler, Moritz, Franzreb, Matthias, Bracewell, Daniel and Thomas, Owen R.T. (2021). Nanoparticle tracking analysis as a process analytical tool for characterising magnetosome preparations. Food and Bioproducts Processing, 127 , pp. 426-434.
Abstract
Nanoparticle Tracking Analysis (NTA) has been employed to measure the particle concentration and size distribution of magnetosomes extracted and purified from Magnetospirillum gryphiswaldense MSR-1, and then exposed to probe ultrasonication for various times, or 1% (w/v) sodium dodecyl sulphate (SDS) for 1 h. Particle concentration increased 3.7-fold over the first 15 min of ultrasonication (from 2 × 108 to >7.3 × 108 particles mL−1), but fell steeply to ∼3.6 × 108 particles mL−1 after 20 min. NTA of untreated magnetosome preparation confirmed a wide particle distribution dominated by larger species (D[1,0] = 312 nm; Dn50 = 261 nm; mode = 243 nm) with no particles in the size range of isolated single magnetosomes. After 5 min of ultrasonication the whole particle size distribution shifted to smaller size (D[1,0] = 133 nm; Dn50 = 99 nm; mode = 36 nm, corresponding to individual magnetosomes), but longer treatment times (15 and 20 min) reversed the previous transition; all characteristic numbers of the particle size distributions increased and very few small particles were detected. Side-by-side comparison of NTA and TEM sizing data revealed remarkable similarity at low ultrasonication times, with both showing single magnetosomes accounted for ∼30% population after 5 min. Exposure of magnetosomes to SDS resulted in a ∼3-fold increase in particle concentration to 5.8 × 108 particles mL−1, narrowing of the size distribution and gross elimination of particles below 60 nm. We conclude that NTA is a rapid cost-effective technique for measuring particle concentration, size distribution and aggregation state of magnetosomes in solution.
Publication DOI: | https://doi.org/10.1016/j.fbp.2021.03.013 |
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Divisions: | College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry College of Engineering & Physical Sciences |
Additional Information: | © 2021, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Uncontrolled Keywords: | Biomanufacturing,Bioprocessing,Magnetic nanoparticles,Nanobiotechnology,Process analytical technology,Quality control,Biotechnology,Food Science,Biochemistry,General Chemical Engineering |
Publication ISSN: | 1744-3571 |
Last Modified: | 11 Nov 2024 08:30 |
Date Deposited: | 07 Apr 2021 12:49 |
Full Text Link: |
https://www.sci ... 960308521000572 |
Related URLs: |
http://www.scop ... tnerID=8YFLogxK
(Scopus URL) |
PURE Output Type: | Article |
Published Date: | 2021-05 |
Published Online Date: | 2021-03-26 |
Accepted Date: | 2021-03-18 |
Authors: |
Fernandez-Castane, Alfred
(
0000-0002-2572-7797)
Overton, Tim W. Li, Hong Joseph, Stephan Ebeler, Moritz Franzreb, Matthias Bracewell, Daniel Thomas, Owen R.T. |
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