Characterisation of osteogenic and vascular responses of hMSCs to Ti-Co doped phosphate glass microspheres using a microfluidic perfusion platform


Using microspherical scaffolds as building blocks to repair bone defects of specific size and shape has been proposed as a tissue engineering strategy. Here, phosphate glass (PG) microcarriers doped with 5 mol % TiO2 and either 0 mol % CoO (CoO 0%) or 2 mol % CoO (CoO 2%) were investigated for their ability to support osteogenic and vascular responses of human mesenchymal stem cells (hMSCs). Together with standard culture techniques, cell-material interactions were studied using a novel perfusion microfluidic bioreactor that enabled cell culture on microspheres, along with automated processing and screening of culture variables. While titanium doping was found to support hMSCs expansion and differentiation, as well as endothelial cell-derived vessel formation, additional doping with cobalt did not improve the functionality of the microspheres. Furthermore, the microfluidic bioreactor enabled screening of culture parameters for cell culture on microspheres that could be potentially translated to a scaled-up system for tissue-engineered bone manufacturing.

Publication DOI:
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
Additional Information: This article is distributed under the terms of the Creative Commons Attribution 4.0 License ( which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages ( Funding: This work was financially supported by an EPSRC Doctoral Training Grant and Industrial Doctorate Centre in Bioprocess Engineering Leadership (grant number: EP/G034656/1); European Union’s Horizon 2020 research and innovation programme, under Grant agreement No 739572; National Research Foundation (NRF), Republic of Korea (NRF2018R1A2B3003446; NRF-2018K1A4A3A01064257). It was also funded in part by the Australian Research Council Discovery Grants Scheme (DP140104217).
Uncontrolled Keywords: Stem cells,microfluidics,osteogenic differentiation,phosphate glass,tissue engineering,Medicine (miscellaneous),Biomaterials,Biomedical Engineering
Publication ISSN: 2041-7314
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Related URLs: https://journal ... 041731420954712 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2020-11-13
Published Online Date: 2020-10-24
Accepted Date: 2020-08-13
Authors: Peticone, Carlotta
Thompson, David De Silva
Dimov, Nikolay
Jevans, Ben
Glass, Nick
Micheletti, Martina
Knowles, Jonathan C
Kim, Hae-Won
Cooper-White, Justin J
Wall, Ivan B (ORCID Profile 0000-0001-6294-8348)



Version: Published Version

License: Creative Commons Attribution

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