Poly-ε-Lysine or Mel4 Antimicrobial Surface Modification on a Novel Peptide Hydrogel Bandage Contact Lens


Microbial keratitis (MK) is a serious issue in many countries and is often caused by contact lens wear. Antimicrobial peptides (AMPs) are a potentially useful tool for creating antimicrobial surfaces in light of increasing antibiotic resistance. Poly-ε-lysine (pεK) is an AMP that has been used extensively as a food preservative and Mel4 has recently been synthesized and studied as an antimicrobial coating for contact lenses. A hydrogel synthesized of pεK cross-linked with biscarboxylic acids provides a potential lens material which has many surface free amines, that can be subsequently used to attach additional AMPs, creating an antimicrobial lens. The aim of this study is to investigate pεK hydrogels against a clinical strain of Pseudomonas aeruginosa (P. aeruginosa) for preventing or treating MK. Covalent attachment of AMPs is investigated and confirmed by fluorescently tagged peptides. Bound pεK effectively reduces the number of adherent P. aeruginosa in vitro (>3 log). In ex vivo studies positive antimicrobial activity is observed on bare pεK hydrogels and those with additionally bound pεK or Mel4; lenses allow the maintenance of the corneal epithelium. A pεK hydrogel contact lens with additional AMPs can be a therapeutic tool to reduce the incidence of MK.

Publication DOI: https://doi.org/10.1002/admi.202001232
Divisions: College of Health & Life Sciences > School of Optometry > Optometry
College of Health & Life Sciences > School of Optometry > Optometry & Vision Science Research Group (OVSRG)
College of Health & Life Sciences
Additional Information: © 2020 The Authors. Published by Wiley‐VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Funding: EPSRC. Grant Number: EP/M002209/1
Uncontrolled Keywords: antimicrobial peptides,contact lens,microbial keratitis,Pseudomonas,Mechanics of Materials,Mechanical Engineering
Publication ISSN: 2196-7350
Last Modified: 11 Dec 2023 08:32
Date Deposited: 30 Sep 2020 12:41
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://onlinel ... /admi.202001232 (Publisher URL)
PURE Output Type: Article
Published Date: 2020-11-01
Published Online Date: 2020-09-21
Accepted Date: 2020-08-14
Authors: Lace, Rebecca
Doherty, Kyle G.
Dutta, Debarun (ORCID Profile 0000-0002-2204-5272)
Willcox, Mark D.P.
Williams, Rachel L.



Version: Published Version

License: Creative Commons Attribution

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