Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Abstract

Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

Publication DOI: https://doi.org/10.1002/smll.v16.3
Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
Additional Information: © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords: biomimetics,drug delivery,extracellular vesicles,gene delivery,microcapsules,nanocapsules,Biotechnology,Biomaterials,Chemistry(all),Materials Science(all)
Publication ISSN: 1613-6810
Full Text Link:
Related URLs: https://onlinel ... /smll.201904880 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2020-01-20
Published Online Date: 2019-12-16
Accepted Date: 2019-10-31
Authors: Tarakanchikova, Yana
Alzubi, Jamal
Pennucci, Valentina
Follo, Marie
Kochergin, Boris
Muslimov, Albert
Skovorodkin, Ilya
Vainio, Seppo
Antipina, Maria N.
Atkin, Vsevolod
Popov, Alexey
Meglinski, Igor (ORCID Profile 0000-0002-7613-8191)
Cathomen, Toni
Cornu, Tatjana I.
Gorin, Dmitry A.
Sukhorukov, Gleb B.
Nazarenko, Irina

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