Rutowska, Monika, Lu, Jiadi, Garcia-Gunning, Fatima C. and Ellis, Andrew D. (2011). DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre. IN: 21st International Conference on Optical Fiber Sensors. Proceedings of SPIE - The International Society for Optical Engineering . CAN: SPIE-Intl Soc Optical Eng.
Abstract
In this paper, we report for the first time the detection of a Cy5-labelled DNA probe immobilised within a 3D hydrogel matrix formed, inside a hollow core Photonic Crystal Fibre (HC-PCF). We show both the sensitivity of fluorescence detection inside the HC-PCF using a supercontinuum light source and of the variation of the luminescence intensity with the concentration DNA probe within the hydrogel. The 3D hydrogel matrix is a network of polymer chains, which is expected to provide highly sensitive detection and selection of bio-molecules, in comparison with 2D coverage. The biocompatibility of hydrogel in the HC-PCF suggests numerous applications associated with immobilised DNA probe detection for point-of-care or remote systems.
| Publication DOI: | https://doi.org/10.1117/12.886070 |
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| Additional Information: | Copyright 2011 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. |
| Event Title: | 21st International Conference on Optical Fiber Sensors |
| Event Type: | Other |
| Event Dates: | 2011-05-15 - 2011-05-19 |
| Uncontrolled Keywords: | biosensing,DNA,fluorescence,hollow core,hydrogel,Photonic crystal fibres,silanisation,Electronic, Optical and Magnetic Materials,Condensed Matter Physics,Computer Science Applications,Applied Mathematics,Electrical and Electronic Engineering |
| ISBN: | 9780819482464 |
| Last Modified: | 15 Sep 2025 17:48 |
| Date Deposited: | 18 Mar 2020 13:53 |
| Full Text Link: | |
| Related URLs: |
http://www.scop ... tnerID=8YFLogxK
(Scopus URL) |
PURE Output Type: | Conference contribution |
| Published Date: | 2011-06-09 |
| Authors: |
Rutowska, Monika
Lu, Jiadi Garcia-Gunning, Fatima C. Ellis, Andrew D. ( 
0000-0002-0417-0547)
|