Influence of Pulsed He–Ne Laser Irradiation on the Red Blood Cell Interaction Studied by Optical Tweezers

Abstract

Optical Tweezers (OT), as a revolutionary innovation in laser physics, has been extremely useful in studying cell interaction dynamics at a single-cell level. The reversible aggregation process of red blood cells (RBCs) has an important influence on blood rheological properties, but the underlying mechanism has not been fully understood. The regulating effects of low-level laser irradiation on blood rheological properties have been reported. However, the influence of pulsed laser irradiation, and the origin of laser irradiation effects on the interaction between RBCs remain unclear. In this study, RBC interaction was assessed in detail with OT. The effects of both continuous and pulsed low-level He–Ne laser irradiation on RBC aggregation was investigated within a short irradiation period (up to 300 s). The results indicate stronger intercellular interaction between RBCs in the enforced disaggregation process, and both the cell contact time and the initial contact area between two RBCs showed an impact on the measured disaggregation force. Meanwhile, the RBC aggregation force that was independent to measurement conditions decreased after a short time of pulsed He–Ne laser irradiation. These results provide new insights into the understanding of the RBC interaction mechanism and laser irradiation effects on blood properties.

Publication DOI: https://doi.org/10.3390/mi10120853
Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
College of Engineering & Physical Sciences
Additional Information: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Funding: China Scholarship Council (CSC No. 201706410089, R.Z.), Tauno Tönning Foundation (grant No. 20190104, R.Z.), EDUFI Fellowship (TM-17-10370, TM-18-10820, T.A.), Suomen Kulttuurirahasto (grant No. 00190188, T.A.), Oulun yliopiston apuraharahasto (No. 20180107, T.A.). I.M. also acknowledges partial support from the Academy of Finland (project 326204, 325097), MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005), and the National Research Tomsk State University Academic D.I. Mendeleev Fund Program.
Uncontrolled Keywords: red blood cells,optical tweezers,RBC interactions,helium-neon laser
Publication ISSN: 2072-666X
Full Text Link:
Related URLs: https://www.mdp ... -666X/10/12/853 (Publisher URL)
PURE Output Type: Article
Published Date: 2019-12-05
Accepted Date: 2019-12-03
Authors: Zhu, Ruixue
Avsievich, Tatiana
Bykov, Alexander
Popov, Alexey
Meglinski, Igor (ORCID Profile 0000-0002-7613-8191)

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