Single Step Plasma Process for Covalent Binding of Antimicrobial Peptides on Catheters to Suppress Bacterial Adhesion


Catheter-associated biofilms are responsible for a large fraction of hospital acquired infections. Antimicrobial surface coating on catheters providing prevention at source is extensively studied to reduce bacterial adhesion. Antimicrobial peptides such as melimine and Mel4, covalently linked to surfaces have shown excellent potential in animal and human studies to suppress infection without toxicity. Covalent binding of the peptides on catheter surfaces improves efficacy but so far has been implemented using multi-step wet chemical coupling that will impede widespread adoption. Here we demonstrate plasma immersion ion implantation (PIII) as a single step treatment that covalently couples antimicrobial peptides to polyvinyl chloride (PVC). Strong antimicrobial activity was demonstrated by higher than 3 log kill of S. aureus. A variant of the process was demonstrated as an antimicrobial treatment for chemically inert glass surfaces. Covalent coupling was rigorously tested by stringent SDS washing. We further demonstrated that the plasma treatment can effectively functionalize both internal and external surfaces of catheter tubing, reducing 99% of bacterial adhesion. The process is feasible as a patient-safe treatment for treating various types of catheters and is suitable for commercial mass production. In a logical extension of the work, the process could be adapted to bone replacement scaffolds of all types including metallic, polymeric and ceramic.

Publication DOI:
Divisions: College of Health & Life Sciences > School of Optometry > Optometry
College of Health & Life Sciences > School of Optometry > Optometry & Vision Science Research Group (OVSRG)
College of Health & Life Sciences
Additional Information: This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Appl. Bio Mater., copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see Funding: Australian Research Council (Grand No. DP170102086), University of New South Wales.
Uncontrolled Keywords: antimicrobial peptide,catheters,melimine,plasma coating,plasma immersion ion implantation,Biomaterials,Chemistry(all),Biomedical Engineering,Biochemistry, medical
Publication ISSN: 2574-0970
Last Modified: 11 Dec 2023 08:24
Date Deposited: 06 Nov 2019 10:07
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Related URLs: ... /acsabm.9b00776 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2019-12-16
Published Online Date: 2019-11-01
Accepted Date: 2019-11-01
Submitted Date: 2019-08-28
Authors: Tran, Clara Thao Hoang
Yasir, Muhamed
Dutta, Debarun (ORCID Profile 0000-0002-2204-5272)
Eswaramoorthy, Nithya
Suchowerska, Natalka
Willcox, Mark
Mckenzie, David R.

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