Optical investigation of three‐dimensional human skin equivalents: A pilot study


Human skin equivalents (HSEs) are three-dimensional living models of human skin that are prepared in vitro by seeding cells onto an appropriate scaffold. They recreate the structure and biological behaviour of real skin, allowing the investigation of processes such as keratinocyte differentiation and interactions between the dermal and epidermal layers. However, for wider applications, their optical and mechanical properties should also replicate those of real skin. We therefore conducted a pilot study to investigate the optical properties of HSEs. We compared Monte Carlo simulations of (a) real human skin and (b) two-layer optical models of HSEs with (c) experimental measurements of transmittance through HSE samples. The skin layers were described using a hybrid collection of optical attenuation coefficients. A linear relationship was observed between the simulations and experiments. For samples thinner than 0.5 mm, an exponential increase in detected power was observed due to fewer instances of absorption and scattering.

Publication DOI: https://doi.org/10.1002/jbio.201960053
Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
College of Engineering & Physical Sciences > Aston Institute of Materials Research (AIMR)
College of Engineering & Physical Sciences
Additional Information: This is the peer reviewed version of the following article: Kallepalli, A, McCall, B, James, DB, Junaid, S, Halls, J, Richardson, MA. Optical investigation of three‐dimensional human skin equivalents: A pilot study. J. Biophotonics. 2019;e20190053, which has been published in final form at https://doi.org/10.1002/jbio.201960053.  This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Uncontrolled Keywords: Monte Carlo,TracePro,lasers,tissue engineering,Chemistry(all),Materials Science(all),Biochemistry, Genetics and Molecular Biology(all),Engineering(all),Physics and Astronomy(all)
Publication ISSN: 1864-0648
Last Modified: 18 Jun 2024 07:23
Date Deposited: 07 Oct 2019 12:22
Full Text Link:
Related URLs: https://onlinel ... /jbio.201960053 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2020-01-01
Published Online Date: 2019-10-08
Accepted Date: 2019-10-03
Authors: Kallepalli, Akhil
McCall, Blake
James, David
Junaid, S (ORCID Profile 0000-0001-9460-710X)
Halls, James
Richardson, Mark



Version: Accepted Version

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